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Updated: Mar 13, 2026

Cell Squeezing as a Robust, Microfluidic Intracellular Delivery Platform
Published on: November 7, 2013
In vitro and ex vivo strategies for intracellular delivery
Martin P Stewart1,2, Armon Sharei1,2,3,4, Xiaoyun Ding1,2
1Department of Chemical Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
Abstract:
Intracellular delivery of materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next-generation systems that can deliver a broad variety of cargo to diverse cell types. Here we review in vitro and ex vivo intracellular delivery approaches with a focus on mechanisms, challenges and opportunities. In particular, we emphasize membrane-disruption-based delivery methods and the transformative role of nanotechnology, microfluidics and laboratory-on-chip technology in advancing the field.
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