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Updated Multiplex PCR for Detection of All Six Plasmid-Mediated qnr Gene Families
Gabriela Bergiante Kraychete1, Larissa Alvarenga Batista Botelho1, Eloiza Helena Campana1
1Laboratório de Investigação em Microbiologia Médica (LIMM), Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Abstract:
Plasmid-mediated qnr genes have been reported in bacteria worldwide and are widely associated with other relevant determinants of resistance in multiresistance plasmids. Here, we provide an update on a previously described multiplex PCR in order to detect all six qnr families (including qnrA, qnrS, qnrB, qnrC, qnrD, and qnrVC) described until now. The proposed method makes possible the screening of these genes, reducing cost and time, and it may demonstrate an underestimated prevalence of the latest variants described.
Insights
This study updates a multiplex PCR method to detect all six known qnr gene families in bacteria. This enhanced screening tool saves time and resources, potentially revealing the true prevalence of these important antimicrobial resistance genes.
Area of Science:
- Microbiology
- Molecular Biology
- Genetics
Background:
- Plasmid-mediated qnr genes are globally prevalent in bacteria.
- These genes are frequently found alongside other resistance determinants on multiresistance plasmids.
- Existing detection methods may not cover all known qnr gene families.
Purpose of the Study:
- To update and validate a multiplex PCR assay for detecting all six described qnr gene families (qnrA, qnrS, qnrB, qnrC, qnrD, and qnrVC).
- To provide a cost-effective and time-efficient method for screening qnr genes in bacterial isolates.
Main Methods:
- Development and validation of an updated multiplex PCR assay.
- Testing the assay's ability to detect all six known qnr gene families.
- Comparative analysis with previous methods to assess efficiency and cost-effectiveness.
Main Results:
- The updated multiplex PCR successfully detects all six qnr gene families.
- The method offers reduced cost and time for screening compared to individual gene detection.
- The assay facilitates the identification of emerging qnr variants.
Conclusions:
- The updated multiplex PCR is a valuable tool for comprehensive surveillance of plasmid-mediated qnr genes.
- This method can help uncover the underestimated prevalence of recently described qnr variants.
- Efficient detection of qnr genes is crucial for understanding and combating antimicrobial resistance.
