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Novel RNA-Binding Proteins Isolation by the RaPID Methodology
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Isolation and characterization of roridin E.

Clark D Ridge1, Eugene P Mazzola1, Martyn P Coles2

  • 1Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, MD, 20740, USA.

Magnetic Resonance in Chemistry : MRC
|October 14, 2016
PubMed
Summary

Roridin E, a potent macrolide from Stachybotrys chartarum, was re-isolated and characterized. Structural analysis revealed discrepancies with existing literature, with crystallographic data providing the first definitive confirmation.

Keywords:
NMR analysisroridin Etrichothecene

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Area of Science:

  • Natural Products Chemistry
  • Mycotoxicology
  • Structural Biology

Background:

  • Stachybotrys chartarum produces mycotoxins, including macrolides like roridin E.
  • Roridin E is known for its high cytotoxicity.
  • Accurate structural identification is crucial for understanding biological activity.

Purpose of the Study:

  • To re-isolate and characterize roridin E from Stachybotrys chartarum.
  • To clarify discrepancies in the existing spectral data for roridin E.
  • To provide definitive structural confirmation using X-ray crystallography.

Main Methods:

  • Re-isolation of roridin E from Stachybotrys chartarum cultures.
  • 1-D and 2-D Nuclear Magnetic Resonance (NMR) spectroscopy for structural elucidation.
  • X-ray crystallographic analysis for absolute structure determination.

Main Results:

  • Roridin E was successfully re-isolated and its structure analyzed.
  • Significant differences were observed between the obtained spectral data and previously reported literature values.
  • X-ray crystallography provided the first unambiguous confirmation of the roridin E structure.

Conclusions:

  • The spectral data presented herein offer a reliable reference for future roridin E identification.
  • The crystallographic data resolves ambiguities and confirms the precise three-dimensional structure of roridin E.
  • This work enhances the understanding of roridin E and aids in its toxicological and pharmacological research.