Related Experiment Video
Updated: Mar 13, 2026
Live-cell Imaging of Lysosomal Membrane Permeabilization During Necroptosis
Published on: November 14, 2025
A light-up imaging protocol for neutral pH-enhanced fluorescence detection of lysosomal neuraminidase activity in
Lei Bao1, Lin Ding1, Jingjing Hui1
1State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, P. R. China. hxju@nju.edu.cn.
Abstract:
A lysosome-accessing nanoprobe is designed for recognition of lysosomal neuraminidases (Lyso-Neus), which can cleave the 4-methylumbelliferone moieties of the substrate from the nanoprobe, and lead to the escape of the moieties from acidic lysosomes into the neutral cytosol assisted by cationic poly(ethyleneimine) to light up the pH-responsive fluorescence for visual detection and dynamic tracking of Lyso-Neu activity in living cells.
