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MicroRNA-183 promotes cell proliferation via regulating programmed cell death 6 in pediatric acute myeloid leukemia

Xiang Wang1, Dongjian Zuo2, Yufang Yuan1

  • 1Department of Pediatrics, Huai'an First People's Hospital, Nanjing Medical University, 6 Beijing Road West, Huai'an, 223300, Jiangsu, China.

Journal of Cancer Research and Clinical Oncology
|October 15, 2016
PubMed
Abstract

Insights

MicroRNA-183 (miR-183) is elevated in pediatric acute myeloid leukemia (AML) and promotes cancer growth by targeting PDCD6. Combined serum miR-183 and PDCD6 levels may predict prognosis in pediatric AML patients.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Pediatric acute myeloid leukemia (AML) remains a significant challenge in pediatric oncology.
  • Understanding the molecular mechanisms driving pediatric AML is crucial for developing effective therapies.
  • MicroRNAs (miRNAs) have emerged as important regulators in various cancers, including AML.

Purpose of the Study:

  • To investigate the role of microRNA (miR)-183 in pediatric acute myeloid leukemia (AML).
  • To identify the target genes of miR-183 and elucidate its functional impact on leukemia cell phenotypes.
  • To evaluate the clinical relevance and prognostic value of miR-183 and its target in pediatric AML.

Main Methods:

  • Real-time quantitative PCR was used to detect miR-183 expression in bone marrow and serum samples from pediatric AML patients and healthy controls.
  • Functional assays were performed to assess the effects of miR-183 on leukemia cell proliferation, apoptosis, and cell cycle.
  • Bioinformatics tools and luciferase reporter assays were employed to predict and validate target genes of miR-183, with programmed cell death 6 (PDCD6) identified as a direct target.

Main Results:

  • miR-183 expression was significantly upregulated in both bone marrow and serum of pediatric AML patients compared to controls.
  • Overexpression of miR-183 promoted leukemia cell proliferation and G1/S transition while inhibiting apoptosis.
  • High serum miR-183 combined with low serum PDCD6 mRNA was associated with specific AML subtypes and unfavorable karyotypes, serving as an independent prognostic factor for survival.

Conclusions:

  • The combination of serum miR-183 and PDCD6 mRNA represents a novel potential prognostic biomarker for pediatric AML.
  • miR-183 functions as an oncogene in pediatric AML, potentially by targeting PDCD6 and promoting cell proliferation.
  • Targeting miR-183 could offer a potential therapeutic strategy for pediatric AML.
Keywords:
Pediatric acute myeloid leukemiaPrognosisProgrammed cell death 6ProliferationmicroRNA-183

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