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Updated: Mar 13, 2026

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Characterization of a new carmovirus isolated from an Adonis plant
Mina Yasaki1, Yasushi Hirano2, Hiroyuki Uga2
1Faculty of Bioresource Sciences, Akita Prefectural University, Shimoshinjo, Akita, 010-0195, Japan.
Abstract:
An isometric virus was isolated from a cultivated Adonis plant (A. ramosa). The purified virus particle is 28 nm in diameter and is composed of a single coat protein and a single RNA genome of 3,991 nucleotides. Sequence analysis showed that the virus is closely related to carnation mottle virus. The virus was used to mechanically infect healthy A. ramosa plants, resulting in mosaic and leaf curl symptoms; however, attempts to inoculate carnation plants did not result in infection. We propose the virus as a new carmovirus and have named it adonis mosaic virus (AdMV).
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