Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Scalable 3D Bioprinting of Human Islets in a Pancreatic Decellularized Extracellular Matrix-Enriched Bioink for Beta-Cell Replacement Therapy.

bioRxiv : the preprint server for biology·2025
Same author

Effect of controlled release of HGF on extracellular vesicle secretion by urine-derived stem cells.

Frontiers in bioengineering and biotechnology·2024
Same author

Intervertebral Disc-on-a-Chip<sup>MF</sup>: A New Model for Mouse Disc Culture via Integrating Mechanical Loading and Dynamic Media Flow.

Advanced materials technologies·2024
Same author

Corrigendum to 'Engineered multilayer ovarian tissue that secretes sex steroids and peptide hormones in response to gonadotropins' [Biomaterials 34/10 (2013) 2412-2420].

Biomaterials·2024
Same author

Neurobiological insights into lower urinary tract dysfunction: evaluating the role of brain-derived neurotrophic factor.

American journal of clinical and experimental urology·2023
Same author

Decellularized human pancreatic extracellular matrix-based physiomimetic microenvironment for human islet culture.

Acta biomaterialia·2023

Related Experiment Video

Updated: Mar 13, 2026

High Throughput Single-cell and Multiple-cell Micro-encapsulation
16:19

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

19.3K

Microfluidic Approach to Cell Microencapsulation.

Varna Sharma1, Michael Hunckler2, Melur K Ramasubramanian1

  • 1Department of Mechanical Engineering, Clemson University, 222 Flour Daniel Building, 216 South Palmetto Blvd., Box 340921, Clemson, SC, 29634-0921, USA.

Methods in Molecular Biology (Clifton, N.J.)
|October 15, 2016
PubMed
Summary
This summary is machine-generated.

A novel microfluidic method offers precise control over alginate capsule formation for islet cell encapsulation, improving Type-1 diabetes treatment potential.

Keywords:
DiabetesIsletsMicroencapsulationMicrofluidic cell encapsulation

More Related Videos

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
06:50

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Published on: February 11, 2019

12.0K
Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

2.5K

Related Experiment Videos

Last Updated: Mar 13, 2026

High Throughput Single-cell and Multiple-cell Micro-encapsulation
16:19

High Throughput Single-cell and Multiple-cell Micro-encapsulation

Published on: June 15, 2012

19.3K
A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms
06:50

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

Published on: February 11, 2019

12.0K
Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses
09:43

Author Spotlight: Unveiling the Polyfunctionality and Heterogeneity in Immune Responses

Published on: March 8, 2024

2.5K

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Diabetes Research

Background:

  • Bioartificial pancreases utilizing insulin-secreting islet cells show promise for Type-1 diabetes treatment.
  • Islet cell microencapsulation in biopolymers is crucial for immunoisolation of transplanted cells.
  • Inconsistent islet cell size and shape hinder uniform microencapsulation, posing a significant challenge.

Purpose of the Study:

  • To present a microfluidic approach for consistent islet cell encapsulation in alginate.
  • To overcome challenges in controlling capsule shape, size, and microstructure during microencapsulation.
  • To enhance the viability of bioartificial pancreas technology for diabetes management.

Main Methods:

  • Utilizing a microfluidic system for controlled alginate droplet generation.
  • Developing a method for uniform microencapsulation of pancreatic islet cells.
  • Characterizing the resulting alginate capsules for size, shape, and structural integrity.

Main Results:

  • Demonstration of a microfluidic technique for reproducible alginate microcapsule production.
  • Achieved consistent control over capsule dimensions and microstructure.
  • Potential for improved immunoisolation and long-term function of encapsulated islets.

Conclusions:

  • The described microfluidic approach offers a viable solution for consistent islet cell microencapsulation.
  • This method addresses key limitations in current encapsulation techniques.
  • It holds significant potential for advancing bioartificial pancreas development for Type-1 diabetes therapy.