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Related Concept Videos

Subviral Agents01:29

Subviral Agents

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Subviral agents are infectious entities that resemble viruses but lack one or more viral components, such as a capsid or essential replication machinery. These agents include viroids, prions, and satellites, each possessing distinct structural and functional characteristics that influence their mode of infection and replication.Viroids are the simplest subviral agents, consisting of circular, single-stranded RNA molecules without a protein coat. They exclusively infect plants, relying entirely...
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Drug Delivery: Miscellaneous Routes01:22

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Drug delivery methods like oral inhalation, nasal sprays, transdermal patches, eye drops, intravitreal injection,  and rectal administration provide localized effects with reduced toxicity.
Oral inhalation and nasal sprays swiftly transfer drugs across the respiratory epithelium's mucosal layer. Inhaled glucocorticoids and bronchodilators directly target lung conditions such as asthma, while fluticasone nasal spray mitigates allergic rhinitis.
Transdermal patches transport drugs...
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Non-viral nucleic acid delivery methods.

Igor Slivac1, David Guay2, Mathias Mangion3

  • 1a Faculty of Food Technology and Biotechnology , University of Zagreb , Zagreb , Croatia.

Expert Opinion on Biological Therapy
|October 15, 2016
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Summary

Non-viral vectors offer safer alternatives for delivering nucleic acids in human cells for treating diseases like cancer. While current methods show moderate success, advanced biological particles hold promise for improving therapeutic efficiency.

Keywords:
Nucleic acid deliveryexosomesparticle mediated transfectionpeptide transduction domainsphysical transfectionvirus derived particles

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Area of Science:

  • Biomedical Engineering
  • Gene Therapy
  • Nanotechnology

Background:

  • Nucleic acid delivery is crucial for treating genetic disorders and cancers.
  • Non-viral vectors are preferred over viral systems due to safety, despite lower efficiency.
  • Physical and vector-assisted methods are key non-viral approaches, each with limitations.

Purpose of the Study:

  • To review non-viral nucleic acid delivery techniques for human cell applications.
  • To highlight recent therapeutic advancements in ex vivo and in vivo delivery.
  • To emphasize the role of biological particles in enhancing delivery efficiency.

Main Methods:

  • Discussion of physical non-viral delivery methods.
  • Analysis of vector-assisted non-viral delivery systems.
  • Focus on biological particles: peptide transduction domains, virus-like particles, gesicles, and exosomes.

Main Results:

  • Non-viral transfection techniques have rapidly increased for human therapy.
  • Current non-viral methods demonstrate moderate success in efficacy.
  • Biological particles show significant potential for improving nucleic acid delivery.

Conclusions:

  • The development of multifunctional hybrid systems is key to matching viral vector efficiency.
  • Biological particles represent a promising avenue for advancing non-viral gene therapy.
  • Optimizing non-viral delivery methods is essential for effective treatment of monogenic diseases and cancers.