Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid procedure for cell colony hybridization using DNA probes.

H Avraham1, S Avraham, S C Bernstein

  • 1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.

Analytical Biochemistry
|June 1, 1989
PubMed
Summary

This study introduces a fast, direct method for screening cell colonies using DNA probes and nitrocellulose filters. It simplifies gene selection in transfected cells, offering a reliable and sensitive approach.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reentrant superconductivity in a naturally occurring Josephson junction array tuned by radio-frequency power.

Nature communications·2026
Same author

Treatment of pediatric ocular rosacea: A systematic review.

Annales de dermatologie et de venereologie·2023
Same author

The Involvement of Lumican in Human Ovulatory Processes.

Reproductive sciences (Thousand Oaks, Calif.)·2021
Same author

Day 5 vitrified blastocyst transfer versus day 6 vitrified blastocyst transfer in oocyte donation program.

Scientific reports·2021
Same author

Graphene Oxide Sheets Combine into Conductive Coatings by Direct Oxidative Electropolymerization.

Scientific reports·2017
Same author

Impaired neurogenesis by HIV-1-Gp120 is rescued by genetic deletion of fatty acid amide hydrolase enzyme.

British journal of pharmacology·2014

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Screening for transfected genes in cell clones is crucial for genetic research.
  • Existing methods can be time-consuming and complex.
  • Efficient selection of desired cell clones is essential for downstream applications.

Purpose of the Study:

  • To develop a rapid and direct screening method for single cell-derived colonies.
  • To enable high-throughput screening of transfected cell populations.
  • To simplify and improve the sensitivity of gene clone selection.

Main Methods:

  • Utilized nitrocellulose filter hybridization.
  • Employed DNA probes for specific gene detection.
  • Applied the technique to screen single cell-derived colonies and foci.

Related Experiment Videos

Main Results:

  • Demonstrated a reliable, rapid, and sensitive screening method.
  • Successfully screened a large number of colonies/foci.
  • Facilitated the selection of cell clones containing the desired transfected gene.

Conclusions:

  • The described method offers a significant improvement over current screening procedures.
  • This technique is effective for selecting cell clones with specific genetic modifications.
  • The method is valuable for molecular biology and genetic engineering applications.