Mixture analysis and quantitative determination of nitrogen-containing organic molecules by surface-enhanced Raman...

Surface-enhanced Raman spectrometry (SERS) on a silver-coated filter paper substrate of nitrogen-containing organic molecules is reported. A correction procedure for standardization of measurements is proposed and evaluated to solve the difficult problem of quantitation of adsorbate in SERS. The relative standard deviation obtained through this procedure is around 15%. Linearity (r = 0.999) was achieved up to 50 micrograms/mL aminoacridine. A limited dynamic range is observed, however, due to the limited number of SERS active sites in the substrate. Spectral fingerprinting of three-component mixtures by concentration-dependent selective molecular adsorption on the substrate is also reported.