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A Simple Mechanical Procedure to Create Limbal Stem Cell Deficiency in Mouse
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[Limbal stem cell deficiency management. A review].

V Kocaba1, O Damour2, C Auxenfans2

  • 1Cornea Center of Excellence, Harvard Medical School, Schepens Eye Research Institute, 20, Staniford Street, Boston, 02114-MA, États-Unis; Department of Ophthalmology, Massachusetts Eye and Ear Infirmary, 243, Charles Street, Boston, 02114-MA, États-Unis; Banque de cornée de Lyon, pavillon I, hôpital Edouard-Herriot, hospices civils de Lyon, 5, place d'Arsonval, 69003 Lyon, France; Service d'ophtalmologie, pavillon C, hôpital Edouard-Herriot, 5, place d'Arsonval, 69003 Lyon, France; Université Claude-Bernard Lyon-I, 43, boulevard du 11-Novembre-1918, 69100 Villeurbanne, France.

Journal Francais D'Ophtalmologie
|October 16, 2016
PubMed
Summary
This summary is machine-generated.

Severe eye burns can cause limbal stem cell deficiency, leading to corneal opacification and blindness. Cultivated limbal or oral mucosal epithelial transplants offer viable treatment alternatives for patients.

Keywords:
Cellular therapyCorneaCornéeDéficit en cellules souches limbiquesLimbal reconstructionLimbal stem cell deficiencyLimbeLimbusReconstruction limbiqueThérapie cellulaire

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Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Stem Cell Biology

Background:

  • Limbal stem cell deficiency (LSCD) often results from severe eye burns, impairing corneal regeneration.
  • This deficiency leads to conjunctivalization, corneal opacification, ulceration, and neovascularization, causing blindness.
  • LSCD disrupts the homeostasis of the entire ocular surface, including conjunctiva, eyelids, and tear film.

Purpose of the Study:

  • To review the evolving management strategies for limbal stem cell deficiency.
  • To highlight advancements in understanding limbal stem cell anatomy and physiology.
  • To present cultivated limbal epithelial and oral mucosal epithelial transplantations as potential therapeutic options.

Main Methods:

  • Review of current literature on limbal stem cell deficiency.
  • Analysis of therapeutic advances in stem cell transplantation for ocular surface reconstruction.
  • Evaluation of cultivated limbal epithelial transplantation (CLET) and oral mucosal epithelial transplantation (OMET) as treatment modalities.

Main Results:

  • Significant progress has been made in understanding LSCD and developing stem cell-based therapies.
  • CLET and OMET have emerged as promising alternatives to traditional treatments.
  • These advanced techniques offer hope for restoring vision in patients with severe ocular surface damage.

Conclusions:

  • Cultivated limbal epithelial and oral mucosal epithelial transplantations represent viable and effective alternatives for treating limbal stem cell deficiency.
  • Continued research and clinical application of these regenerative approaches are crucial for improving patient outcomes.
  • These treatments offer a renewed hope for restoring visual function and quality of life for severely affected individuals.