Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Neonatal Survival After Serial Amnioinfusions for Anhydramnios Due to Fetal Kidney Failure: The RAFT Clinical Trial.

JAMA·2026

Same author

Comparative long-term outcomes of Heller myotomy and peroral endoscopic myotomy.

Surgical endoscopy·2026

Same author

Understanding the Barriers to Translating Artificial Intelligence into the Clinical Laboratory.

European journal of internal medicine·2026

Same author

Impact of Concomitant Mitral Regurgitation in Moderate Aortic Stenosis: Assessment of Remodeling and Clinical Outcomes.

JACC. Cardiovascular imaging·2026

Same author

Intercostal Nerve Cryoablation and Pain Outcomes in Robotic Lung Surgery.

Annals of thoracic surgery short reports·2026

Same author

Long-Term Outcomes of Renal Transplant Allografts With De Novo DSA in the Setting of Stable Function.

Clinical transplantation·2026

Search research articles

Related Experiment Video

Updated: Mar 13, 2026

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Published on: January 29, 2016

Differentiating between live and dead Mycobacterium smegmatis using autofluorescence.

Cynthia Wong¹, Ngan P Ha², Michal E Pawlowski¹

¹Department of Bioengineering, Rice University, 6500 Main Street, Houston, TX 77005, United States.

Tuberculosis (Edinburgh, Scotland)

|October 16, 2016

Summary

Monitoring tuberculosis (TB) treatment response is crucial, especially for drug-resistant strains. Researchers found that live and dead mycobacteria exhibit different photobleaching rates of Coenzyme F420, offering a potential new method for treatment monitoring.

Keywords:

Autofluorescence Microscopy Mycobacterium Viability

More Related Videos

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Published on: September 5, 2013

Optimized Workflow for Iterative Bleaching Extends Multiplexity Imaging of Highly Autofluorescent Clinical Samples

Optimized Workflow for Iterative Bleaching Extends Multiplexity Imaging of Highly Autofluorescent Clinical Samples

Published on: July 11, 2025

Related Experiment Videos

Last Updated: Mar 13, 2026

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Molecular Probe Optimization to Determine Cell Mortality in a Photosynthetic Organism Microcystis aeruginosa Using Flow Cytometry

Published on: January 29, 2016

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Published on: September 5, 2013

Optimized Workflow for Iterative Bleaching Extends Multiplexity Imaging of Highly Autofluorescent Clinical Samples

Optimized Workflow for Iterative Bleaching Extends Multiplexity Imaging of Highly Autofluorescent Clinical Samples

Published on: July 11, 2025

Area of Science:

Microbiology
Biochemistry
Medical Diagnostics

Background:

Current tuberculosis (TB) diagnostics and treatment monitoring methods have limitations, particularly for multi-drug resistant (MDR) and extensively drug-resistant (XDR) TB.
Culture-based methods for verifying mycobacterial viability are slow (up to six weeks), potentially leading to unnecessary treatment costs and host-drug toxicity.
There is a need for rapid and efficient methods to monitor patient response to TB treatment.

Purpose of the Study:

To investigate the potential of Coenzyme Factor 420 (F420) photobleaching rates as a novel method for monitoring TB treatment response.
To determine if live and dead mycobacteria exhibit differential photobleaching characteristics of F420.

Main Methods:

Utilized Mycobacterium smegmatis as a model organism.
Measured the photobleaching rates of Coenzyme F420 in live and dead mycobacteria over a 2-minute period.
Analyzed the differences in photobleaching kinetics between live and dead bacterial populations.

Main Results:

Preliminary experiments demonstrated that live and dead Mycobacterium smegmatis undergo distinct rates of F420 photobleaching.
These observed differences in photobleaching kinetics suggest a potential biomarker for mycobacterial viability.

Conclusions:

The differential photobleaching of Coenzyme F420 by live and dead mycobacteria presents a promising avenue for developing faster TB treatment monitoring tools.
Further studies are warranted to validate these findings with Mycobacterium tuberculosis (Mtb) and assess clinical applicability.