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Evaluation of multi-allergen dipstick screening test
J T Twiggs1, R L Gray, K Pichler
1Department of Allergy, Marshfield Clinic, Wisconsin.
Annals of Allergy
|September 1, 1989
Summary
The Quidel Allergen Screen (QAS) accurately detects specific serum IgE, matching the performance of Phadebas RAST. This allergy test offers a rapid, in vitro diagnostic method for atopic sensitivity.
Area of Science:
- Clinical immunology
- Allergen diagnostics
- In vitro diagnostics
Background:
- Atopic sensitivity diagnosis relies on skin prick tests, intradermal tests, and serum IgE detection.
- Phadebas RAST is a common method for quantifying specific IgE antibodies.
- There is a need for rapid and easily interpretable in vitro allergy diagnostic methods.
Purpose of the Study:
- To evaluate the diagnostic performance of the Quidel Allergen Screen (QAS).
- To compare the QAS with the Phadebas RAST and clinical assessment for detecting specific serum IgE.
- To determine if QAS is a sensitive and specific method for allergy diagnosis.
Main Methods:
- 43 patients with known atopic sensitivity were tested.
- Quidel Allergen Screen (QAS) and Phadebas RAST were performed in parallel.
- Results were compared against standard skin prick tests, intradermal tests, and clinical assessment.
Main Results:
- The Quidel Allergen Screen (QAS) demonstrated comparable sensitivity and specificity to Phadebas RAST.
- No statistically significant differences were found between QAS and RAST.
- QAS provided results that aligned with clinical assessments.
Conclusions:
- The Quidel Allergen Screen (QAS) is a valid in vitro method for detecting specific serum IgE.
- QAS offers a rapid and easily interpretable alternative to Phadebas RAST for allergy diagnostics.
- QAS shows potential as an effective tool in diagnosing atopic sensitivity.