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In vitro evaluation of pyrethroid-mediated changes on neuronal burst parameters using microelectrode arrays
Baskar Mohana Krishnan1, Balakrishna Murthy Prakhya1
1Department of Toxicology, International Institute of Biotechnology and Toxicology (IIBAT), Padappai-601301, Tamil Nadu, India.
Neurotoxicology
|October 18, 2016
Summary
Pyrethroids alter neuronal network burst activity, with mean burst rate (MBR) most affected. Type-2 pyrethroids are more potent than type-1, impacting MBR, mean interburst interval (MIBI), and percent spikes in burst (PSB).
Area of Science:
- Neuroscience
- Toxicology
- Pharmacology
Background:
- Pyrethroids are widely used insecticides with known neurotoxic effects.
- Neuronal network activity, characterized by burst parameters, is a sensitive indicator of neurotoxicity.
- Understanding pyrethroid effects on neuronal function is crucial for risk assessment.
Purpose of the Study:
- To investigate the impact of six pyrethroids on rat cortical neuronal network burst parameters.
- To determine the relative potency of different pyrethroids based on their effects on neuronal activity.
- To differentiate between type-1 and type-2 pyrethroids' neurotoxic mechanisms.
Main Methods:
- Utilized the microelectrode array technique to record neuronal activity.
- Exposed rat cortical neuronal networks (24-35 DIV) to five concentrations of beta-cyfluthrin, bifenthrin, cypermethrin, deltamethrin, lambda-cyhalothrin, and permethrin.
- Analyzed changes in burst parameters including mean burst rate (MBR), percent spikes in burst (PSB), mean burst duration (MBD), mean spikes in burst (MSB), mean interspike interval in burst (MISIB), and mean interburst interval (MIBI).
Main Results:
- Mean burst rate (MBR), mean interburst interval (MIBI), and percent spikes in burst (PSB) showed the most significant changes at initial pyrethroid concentrations.
- Mean spikes in burst (MSB), mean burst duration (MBD), and mean interspike interval in burst (MISIB) were less affected by initial concentrations.
- Type-2 pyrethroids (beta-cyfluthrin, cypermethrin, deltamethrin, lambda-cyhalothrin) demonstrated higher potency than type-1 pyrethroids (bifenthrin, permethrin) in altering burst parameters.
- A consistent pattern showed MBR increasing at lower concentrations and decreasing at higher concentrations, accompanied by a drop in MIBI.
Conclusions:
- Pyrethroids significantly modulate neuronal network burst activity, with MBR being the most sensitive parameter.
- The study successfully distinguished between the neurotoxic potencies of type-1 and type-2 pyrethroids.
- Findings highlight the differential effects of pyrethroids on neuronal function, informing toxicological assessments.
