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Published on: April 25, 2025
Development of a real-time PCR for Bartonella spp. detection, a current emerging microorganism
Elena Parra1, Ferran Segura1, Jessica Tijero1
1Infectious Diseases Department, Corporació Sanitària Parc Taulí, Institut de Investigació I Innovació Sanitària Parc Taulí, Universitat Autònoma de Barcelona, Parc Taulí 1, Sabadell 08208, Barcelona, Spain.
Abstract:
A real-time PCR assay using SYBR Green was optimized to detect those Bartonella that are most frequently described as pathogens. The assay was genus-specific. Sequencing allowed to distinguish species. Assay sensitivity was determined using 10-fold serial dilutions of genomic DNA. Dynamic range was 100 ng-100 fg and sensitivity was 50 copies/reaction.

