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Superoxide anion scavenging activity of alk(en)yl phenol compounds by using PMS-NADH system

Noriyoshi Masuoka¹, Kulwadee Tamsampaoloet², Warinthorn Chavasiri²

¹Department of Life Science, Okayama University of Science, Okayama, Japan; Research and Development Division of Meister Bio Co. Ltd., Incubator, Okayama University, 1-1-1 Tsushimanaka, Kita-ku, Okayama 700-0082, Japan.

|October 18, 2016

Abstract:

Anacardic acid C_15:3 and cardol C_15:3 sigmoidally suppressed superoxide anion (O₂-) generation using xanthine oxidase. To study this suppression activity, anacardic acids, cardanols and cardols having different numbers of double bonds in alk(en)yl chains were prepared. The O₂- scavenging activity and H₂O₂ formation from O₂- using PMS-NADH were examined. Anacardic acids and cardols indicated sigmoidal O₂- scavenging activity but cardanols did not. The O₂- scavenging activity of anacardic acid C_15:3 was weaker than the suppression activity using xanthine oxidase, but the scavenging activity of cardol C_15:3 was quite similar to the suppression using xanthine oxidase. The H₂O₂ formation from O₂- decreased by the addition of anacardic acids, cardanols and cardols but increased by the addition of gallic and caffeic acids. From these results, we deduced that the O₂- suppression activity of xanthine oxidase reaction with cardols is the O₂- scavenging activity and that anacardic acids and cardols are O₂- scavengers having low prooxidant property.

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