Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gastrulation01:56

Gastrulation

68.4K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
68.4K
Neurulation01:30

Neurulation

47.0K
Neurulation is the embryological process which forms the precursors of the central nervous system and occurs after gastrulation has established the three primary cell layers of the embryo: ectoderm, mesoderm, and endoderm. In humans, the majority of this system is formed via primary neurulation, in which the central portion of the ectoderm—originally appearing as a flat sheet of cells—folds upwards and inwards, sealing off to form a hollow neural tube. As development proceeds, the...
47.0K
Cleavage and Blastulation01:33

Cleavage and Blastulation

51.0K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
51.0K
Determination01:51

Determination

21.3K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
21.3K
Development of the Sexual Organs in the Embryo and Fetus01:15

Development of the Sexual Organs in the Embryo and Fetus

4.5K
Development of the reproductive organs in an embryo starts from a bipotential state. This means the early embryo can develop either male or female reproductive organs. The formation of these organs begins with the growth of gonadal ridges that arise from the intermediate mesoderm during the fifth week of development.
Near the gonadal ridges, two duct systems are present: the mesonephric ducts (Wolffian ducts) and paramesonephric ducts (Müllerian ducts). These ducts form the basis for the...
4.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ectopic mesodermal expression promoted by the eight-cell stage Bufo arenarum subequatorial cytoplasm.

Development genes and evolution·1999
Same author

Ectoderm promotes differentiation of the presumptive ventral mesoderm in early gastrulae of Bufo arenarum.

The Journal of experimental zoology·1993
Same author

Mechanisms underlying the organizer formation in Bufo arenarum embryos.

Microscopia electronica y biologia celular : organo oficial de las Sociedades Latinoamericana de Microscopia Electronica e Iberoamericana de Biologia Celular·1989
Same author

Spermatolysins in Bufo arenarum: their activity on oocyte surface.

The Journal of experimental zoology·1989
Same author

A fast and reliable celloidin-paraffin embedding technique for yolked amphibian embryos.

Mikroskopie·1983
Same author

Easy preparation of stainless steel microdissection instruments by an improved electrical etching technique.

Mikroskopie·1983

Related Experiment Video

Updated: Mar 13, 2026

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
12:59

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

4.2K

Inductive interactions required for mesodermal differentiation in Bufo arenarum gastrulae.

M E Manes1, Fernando Campos Casal1

  • 1Cátedra de Histología y Embriología, Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán, 4000, Tucumán, Argentina, , , , , , AR.

Development Genes and Evolution
|October 18, 2016
PubMed
Summary
This summary is machine-generated.

The dorso-marginal epithelium in Bufo arenarum acts as a potent dorsal organizer. This tissue induces axial structures, including notochord and muscle, from surrounding mesodermal cells in early embryonic development.

Keywords:
Key words amphibian morphogenesisdifferentiationdorsalizationmesodermorganizer

More Related Videos

Generation of Naïve Blastoderm Explants from Zebrafish Embryos
07:21

Generation of Naïve Blastoderm Explants from Zebrafish Embryos

Published on: July 30, 2021

4.0K
Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
11:48

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Published on: January 17, 2016

9.4K

Related Experiment Videos

Last Updated: Mar 13, 2026

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation
12:59

Three and Four-Dimensional Visualization and Analysis Approaches to Study Vertebrate Axial Elongation and Segmentation

Published on: February 28, 2021

4.2K
Generation of Naïve Blastoderm Explants from Zebrafish Embryos
07:21

Generation of Naïve Blastoderm Explants from Zebrafish Embryos

Published on: July 30, 2021

4.0K
Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression
11:48

Grafting of Beads into Developing Chicken Embryo Limbs to Identify Signal Transduction Pathways Affecting Gene Expression

Published on: January 17, 2016

9.4K

Area of Science:

  • Developmental Biology
  • Embryology
  • Cell Biology

Background:

  • The early gastrula stage is critical for establishing embryonic axes.
  • The dorso-marginal epithelium's role as an organizer has been previously suggested but requires detailed investigation.

Purpose of the Study:

  • To investigate the dorsalizing activity of the dorso-marginal epithelium in Bufo arenarum.
  • To elucidate the mechanisms by which this epithelium induces mesodermal differentiation.

Main Methods:

  • Explant cultures of dorso-marginal epithelium were performed.
  • FDA (fluorescein-dextran-amine)-labeled explants were used to trace cell origins.
  • Bisection of the dorso-marginal epithelium in whole embryos was conducted.

Main Results:

  • Dorso-marginal epithelium explants induced notochord formation from ventro-mesodermal cells.
  • Co-culture with ectoderm led to diverse dorso-lateral structures (notochord, muscle, nephric tubules, mesothelium, mesenchyme).
  • Cell contact between the epithelium and mesodermal cells was crucial for extensive mesodermal development; without it, only erythrocytes formed.

Conclusions:

  • The dorso-marginal epithelium functions as a primary dorsal organizer in Bufo arenarum.
  • This tissue possesses potent inductive capabilities, directing mesodermal cell fate and axial patterning.
  • Cell-cell interactions are essential for the full spectrum of inductive events mediated by this epithelium.