Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Migration01:19

Cell Migration

7.2K
Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
7.2K
Cell Migration01:09

Cell Migration

19.0K
Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
19.0K
Role of Myosin in Cell Migration01:18

Role of Myosin in Cell Migration

3.6K
Myosins are multimeric motor proteins involved in various cellular processes such as migration, adhesion, and proliferation. Myosin II is the most common type in animal cells, which binds and cross-links actin filaments.
Myosin II  is a hexamer comprising two heavy chains with globular heads and coiled-coil tails, two regulatory light chains, and two essential light chains. The ATPase sites on the myosin heads hydrolyze ATP, and the released phosphate generates the force for contraction....
3.6K
Cytoskeletal Coordination in Cell Migration01:32

Cytoskeletal Coordination in Cell Migration

5.7K
A migrating cell changes its shape during the cyclic events of attachment and detachment from the substratum and repositions the cell organelles correspondingly. These complex events are orchestrated by the dynamic cytoskeletal network comprising actin filaments, intermediate filaments, and microtubules. Cytoskeletal crosstalk — the direct and indirect communication between the different components — is crucial for this coordination. Direct communication involves various linker...
5.7K
Cell Motility through Blebbing01:16

Cell Motility through Blebbing

2.6K
Blebs are a type of membrane protrusion formed by the internal hydrostatic pressure of the cytoplasm. Blebs are observed in several cell types, including fibroblasts, immune cells, and single-celled organisms like the amoeba. The primary function of blebs is cell locomotion and apoptosis, but they are also found during necrosis and cell division. The life cycle of a bleb comprises an initiation phase followed by the expansion and retraction phases.
Blebbing Through the Matrix
In multicellular...
2.6K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

7.0K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
7.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Effects of Intracellular Force Localization on Cancer Cell Invasion: Revealing Mechanical Trade-offs through Experimentally Validated Computational Models.

ACS biomaterials science & engineering·2026
Same author

Moisture-Responsive Friction Adaptability: Rethinking the Conventional Skin Silicone Interfaces in Pressure Injury Prevention Dressing Designs.

International wound journal·2026
Same author

Moisture-Responsive Thermal Conductivity Properties of Hydrofiber Versus Polyurethane Foam: Implications for Pressure Injury Prevention.

International wound journal·2026
Same author

Rheological Assessment for Determining Form Stability of Wound Dressings.

International wound journal·2025
Same author

Laboratory Evaluations of Wound Dressings: Key Advances to Reflect Clinical Reality.

International wound journal·2025
Same author

Start your engines: How migratory fibroblasts respond to and remember mechanical stretch.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same journal

Economic Impact of Negative Pressure Wound Therapy With Instillation Compared With Standard Negative Pressure Wound Therapy and Gauze Dressing in Acute Traumatic Wounds.

International wound journal·2026
Same journal

Investigation of the Incidence, Risk Factors and Characteristics of Respiratory Device-Related Pressure Injuries.

International wound journal·2026
Same journal

Immunoinflammatory Profile of FGF-18, IL-35 and Glutamic Acid Decarboxylase in Patients With Diabetic Foot Ulcers.

International wound journal·2026
Same journal

Burden and Associated Factors of Diabetic Foot Complications in a Tertiary Referral Cohort Between 2021 and 2024 in the Qassim Region, Saudi Arabia: A Retrospective Cross-Sectional Study.

International wound journal·2026
Same journal

Malignancy in Chronic Leg Wounds: Diagnostic Delay and Clinical Implications in a Tertiary Wound-Care Cohort.

International wound journal·2026
Same journal

Compliance With Foot Care Practices Among Patients With Diabetes Mellitus in Sudan.

International wound journal·2026
See all related articles

Related Experiment Video

Updated: Mar 13, 2026

Stretching Micropatterned Cells on a PDMS Membrane
09:41

Stretching Micropatterned Cells on a PDMS Membrane

Published on: January 22, 2014

16.0K

Low-level stretching accelerates cell migration into a gap.

Samer Toume1, Amit Gefen2, Daphne Weihs1

  • 1Faculty of Biomedical Engineering, Technion-Israel Institute of Technology, Haifa, Israel.

International Wound Journal
|October 18, 2016
PubMed
Summary
This summary is machine-generated.

Low-level (3%) radial stretching of cell monolayers significantly enhances their migration rate to close gaps, accelerating wound healing. This controlled mechanical strain shows promise for optimizing therapeutic interventions.

Keywords:
Cell migrationGap closureMechanobiologySustained deformationsWound healing

More Related Videos

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
05:50

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

Published on: November 1, 2021

2.9K
Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
11:43

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Published on: April 3, 2015

9.0K

Related Experiment Videos

Last Updated: Mar 13, 2026

Stretching Micropatterned Cells on a PDMS Membrane
09:41

Stretching Micropatterned Cells on a PDMS Membrane

Published on: January 22, 2014

16.0K
Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy
05:50

Measuring Cell-Edge Protrusion Dynamics during Spreading using Live-Cell Microscopy

Published on: November 1, 2021

2.9K
Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration
11:43

Concentric Gel System to Study the Biophysical Role of Matrix Microenvironment on 3D Cell Migration

Published on: April 3, 2015

9.0K

Area of Science:

  • Cell biology
  • Biophysics
  • Tissue engineering

Background:

  • Wound healing is a complex biological process involving cell migration.
  • External mechanical forces, like negative pressure wound therapy, can accelerate healing in vivo.
  • The direct impact of mechanical deformations on cell migration during wound closure remains under investigation.

Purpose of the Study:

  • To investigate the effect of sustained, radially applied tensile strain on the migration of cell monolayers during gap closure.
  • To quantify the relationship between mechanical strain and cell migration kinematics.

Main Methods:

  • Murine fibroblast monolayers were cultured on stretchable substrates.
  • A custom 3D-printed apparatus applied sustained tensile strains (3% and 6%).
  • Monolayers were wounded, and cell migration during gap closure was monitored and analyzed.

Main Results:

  • A 3% tensile strain significantly increased normalized cell migration rates and reduced gap closure time compared to controls.
  • A 6% strain did not show the same enhancement, suggesting a strain-dependent effect.
  • Initial gap area showed a linear correlation with maximum migration rate, particularly under applied strain.

Conclusions:

  • Low-level mechanical stretching (3%) enhances en masse cell migration, a key factor in wound healing.
  • Sustained tensile strain can be a tunable parameter to optimize wound healing processes.
  • Findings suggest potential applications in refining therapeutic strategies for wound management.