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Dynamics of the mouse brain cortical synaptic proteome during postnatal brain development
Miguel A Gonzalez-Lozano1, Patricia Klemmer1, Titia Gebuis1
1Department of Molecular and Cellular Neurobiology, Center for Neurogenomics &Cognitive Research, Neuroscience Campus Amsterdam, VU University, Amsterdam, The Netherlands.
Scientific Reports
|October 18, 2016
Summary
Brain synapse development involves significant proteome changes. This study reveals temporal protein abundance shifts during maturation, impacting synaptic function and density.
Area of Science:
- Neuroscience
- Proteomics
- Molecular Biology
Background:
- Synapse development is crucial for brain maturation, involving complex proteomic alterations.
- Previous studies have sparsely investigated the synaptic proteome during development.
Purpose of the Study:
- To comprehensively analyze the temporal changes in the cortical synaptic membrane proteome during mouse brain development.
- To identify key proteins and pathways involved in synapse maturation and function.
Main Methods:
- Quantitative proteomics workflow applied to cortical synaptic membranes from mice at various postnatal ages (P9-P280).
- Statistical analysis to identify proteins with significant temporal abundance changes.
Main Results:
- Quantified 1560 proteins, with 696 showing significant temporal differences.
- Synaptic proteins increased with maturation; protein synthesis proteins decreased.
- Specific proteins like NMDA receptor subunits and SNARE proteins exhibited distinct temporal regulation patterns.
- Cxadr knockdown in neurons significantly reduced synapse density.
Conclusions:
- Synapse maturation is characterized by dynamic proteomic remodeling.
- Temporal regulation of specific protein groups, including synaptic and SNARE proteins, is critical for neuronal development.
- Cxadr plays a functional role in regulating synapse density during development.
