Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Mar 13, 2026

A Step by Step Protocol for Subretinal Surgery in Rabbits
12:31

A Step by Step Protocol for Subretinal Surgery in Rabbits

Published on: September 13, 2016

16.1K

Subchoroidal Release of VEGF and bFGF Produces Choroidal Neovascularization in Rabbit.

Corinne G Wong1, Mehran Taban2, Kathryn Osann3

  • 1a SCLERA LLC , Carlsbad , CA , USA.

Current Eye Research
|October 18, 2016
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ureteral Access Sheath Deployment: Understanding the Force Tolerance of the Human Ureter and the Development of Ureteral Injury During Endoscopic Surgery.

Journal of endourology·2025
Same author

Retinal and Optic Nerve Lesions Correspond to Amyloid in Autosomal Dominant Alzheimer's Disease.

medRxiv : the preprint server for health sciences·2025
Same author

Clinical determination of the natural distensibility of the human ureter: initial study.

BJU international·2024
Same author

Selective, Temporary Postoperative Inhibition of Lymphangiogenesis by Integrin α5β1 Blockade Improves Allograft Survival in a Murine Model of High-Risk Corneal Transplantation.

Journal of clinical medicine·2024
Same author

The growing research toolbox for SLC13A5 citrate transporter disorder: a rare disease with animal models, cell lines, an ongoing Natural History Study and an engaged patient advocacy organization.

Therapeutic advances in rare disease·2024
Same author

Expression of the plasma membrane citrate carrier (pmCiC) in human cancerous tissues-correlation with tumour aggressiveness.

Frontiers in cell and developmental biology·2024

Sustained subchoroidal release of vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) rapidly induced choroidal neovascularization (CNV) in rabbits. This finding may inform novel therapeutic strategies for CNV.

Area of Science:

  • Ophthalmology
  • Vascular Biology
  • Regenerative Medicine

Background:

  • Intravitreal vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) are known to cause retinal neovascularization and hemorrhage.
  • Choroidal neovascularization (CNV) is a significant cause of vision loss.

Purpose of the Study:

  • To determine if sustained subchoroidal release of VEGF and bFGF can induce robust choroidal neovascularization (CNV) in rabbits.
  • To establish a rabbit model for studying CNV development and potential therapies.

Main Methods:

  • Subchoroidal implantation of polymeric pellets containing VEGF and bFGF in Dutch belted and NZW albino rabbits.
  • Control groups received blank pellets without growth factors.
  • Weekly monitoring using ophthalmoscopy, fundus photography, and fluorescein angiography over 4 weeks.
Keywords:
Basic fibroblast growth factorrobust rabbit CNV modelsubchoroidal sustained releasetransscleral implantationvascular endothelial growth factor

More Related Videos

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye
11:49

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

Published on: August 16, 2014

17.6K
A Mouse Model for Laser-induced Choroidal Neovascularization
07:08

A Mouse Model for Laser-induced Choroidal Neovascularization

Published on: December 27, 2015

19.0K

Related Experiment Videos

Last Updated: Mar 13, 2026

A Step by Step Protocol for Subretinal Surgery in Rabbits
12:31

A Step by Step Protocol for Subretinal Surgery in Rabbits

Published on: September 13, 2016

16.1K
The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye
11:49

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

Published on: August 16, 2014

17.6K
A Mouse Model for Laser-induced Choroidal Neovascularization
07:08

A Mouse Model for Laser-induced Choroidal Neovascularization

Published on: December 27, 2015

19.0K
  • Histologic and immunohistochemical analyses, along with in vitro release assays (ELISA).

    • Main Results:

    • All eyes with VEGF/bFGF implants showed significant fluorescein leakage by weeks 2-4, indicating CNV.
    • Histologic analysis confirmed new subretinal blood vessel growth in experimental eyes.
    • Control groups with blank implants exhibited no fluorescein leakage or vascular changes.
    • In vitro studies confirmed sustained release of VEGF and bFGF from the implants.

    Conclusions:

    • Sustained subchoroidal delivery of VEGF and bFGF effectively produces experimental CNV in rabbits.
    • This model provides a platform for understanding CNV pathogenesis.
    • Insights gained may lead to novel therapeutic strategies for treating CNV.