[Features of glia growth in tissue cultures of the rat cerebrum]

In the tissue culture of the newborn rat cerebrum structural peculiarities of the glial growth have been investigated, using scanning and transmissive microscopy. Glioblasts are the first to migrate into the growth zone from the explant. Formation of the glial network is accompanied with formation of multilayered structures. In the cerebral tissue culture 3 zones are distinguished differing from each other by their morphofunctional peculiarities and behavior of the glial cells: growth zone, intermediate and central ones. The growth zone is characterized with permanent moving and modification of glial cells, participating in formation of cellular cords, networks and layers. Glioblasts are predominate cells in this zone. The intermediate zone consists of multilayered glial elements, possessing a high proliferative activity. The central zone, where neuropil and neurons consisting of numerous processes are situated, is characterized with a high degree of differentiation of glial elements. The central part of the explants is covered with epitheliomorphic layer of glial cells, predominantly consisting of cytoplasmic and fibrous astrocytes. The data presented demonstrate that differentiation of glial elements into various types occurs in the cerebral cultures exclusively in the area of the neural cells localization and, possibly, under their immediate influence.