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Random-access scanning microscopy for 3D imaging in awake behaving animals.

K M Naga Srinivas Nadella¹, Hana Roš¹, Chiara Baragli¹

¹Department of Neuroscience, Physiology and Pharmacology, University College London, London, United Kingdom.

|November 8, 2016

Summary

This study introduces a fast acousto-optic lens two-photon microscope for high-speed 3D neural circuit imaging. The technology enables precise, random-access recording from specific neurons in awake mice.

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Area of Science:

Neuroscience
Optical Imaging
Microscopy

Background:

Neural circuits are complex, requiring advanced tools for activity measurement.
Understanding information processing necessitates rapid, spatially resolved neuronal recordings.

Purpose of the Study:

To develop a high-speed, 3D-capable acousto-optic lens two-photon microscope.
To demonstrate random-access imaging for studying neural activity in vivo.

Main Methods:

Acousto-optic lens two-photon microscopy for high-speed focusing and scanning.
Random-access imaging of sparsely distributed neurons in cerebellum and neocortex.
Simultaneous recording from different neuronal compartments (soma, dendrites) in awake mice.

Main Results:

The microscope achieves high-speed focusing and line scanning within a hundreds-of-micrometers volume.
Demonstrated selective imaging of cerebellar interneurons in 3D.
Successfully recorded activity from soma and dendrites of neocortical pyramidal cells simultaneously.

Conclusions:

The acousto-optic lens microscope offers a powerful tool for rapid, 3D neural circuit analysis.
Enables precise, targeted imaging of neuronal populations and subcellular structures in behaving animals.
Advances the study of neural information processing in complex brain structures.