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A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells
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Distinction between breast cancer cell subtypes using third harmonic generation microscopy.

Evangelia Gavgiotaki1,2, George Filippidis1, Haris Markomanolaki2

  • 1Institute of Electronic Structure and Laser, Foundation for Research and Technology, Heraklion, 71110, Crete, Greece.

Journal of Biophotonics
|October 19, 2016
PubMed
Summary

Third Harmonic Generation (THG) microscopy non-invasively images lipid profiles in breast cancer cells. This label-free technique distinguishes HER2-positive cells and links lipid signals to chemical information.

Keywords:
FTIR spectroscopyThird Harmonic Generationbreast cancer cellslinkagelipid dropletsmicroscopynonlinear imagingquantification

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Area of Science:

  • Biomedical Optics
  • Cancer Cell Biology
  • Spectroscopy

Background:

  • Third Harmonic Generation (THG) microscopy is a label-free imaging technique.
  • Lipid profiles are crucial in understanding cancer cell behavior.
  • Distinguishing cancer subtypes non-invasively is a significant challenge.

Purpose of the Study:

  • To investigate the utility of THG microscopy for analyzing lipid profiles in breast cancer cells.
  • To correlate THG signals with specific cancer cell characteristics, such as HER2 expression.
  • To link THG imaging with chemical information obtained from Fourier transform infrared (FTIR) spectroscopy.

Main Methods:

  • Utilized THG microscopy for non-invasive, label-free imaging of breast cancer cells.
  • Quantified THG signals originating from lipid droplets and membrane lipid bilayers.
  • Performed Fourier transform infrared (FTIR) spectroscopy analysis on the same cells.
  • Correlated THG signal intensity and spectral data with HER2 expression levels.

Main Results:

  • THG signal intensity accurately differentiated HER2-positive breast cancer cells from others.
  • THG signals primarily originated from lipid droplets and membrane lipid bilayers.
  • FTIR spectra revealed cancer-specific lipid profiles.
  • A correlation was established between lipid raft-associated FTIR spectra and THG signals, linking imaging to chemical composition.

Conclusions:

  • THG microscopy offers a non-invasive, label-free method to assess lipid profiles in breast cancer cells.
  • THG imaging can distinguish between different breast cancer subtypes, specifically HER2-positive cells.
  • Combining THG microscopy with FTIR spectroscopy provides valuable chemical insights into cancer cell lipids.