Effects of craniofacial structures on mouse palatal closure in vitro

Heads of Swiss-Webster mouse fetuses of four ages spanning days 12-13 of gestation, were partially dissected by removing the brain (B), tongue (T) and mandible (M) alone or in combination (BT, BM, BTM). Preparations were suspended in a gassed, circulating culture system such that palatal closure must take place against gravity. Closure occurred earlier than in vivo and required the posterior half of the mandible be intact and the tongue removed.