Changes in the Dermal Structure during Cultured Epidermal Autograft Engraftment Process
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Published on: June 29, 2022
Minoru Hayashi1, Hideyuki Muramatsu1, Minoru Nakano1
1Department of Plastic and Reconstructive Surgery, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Advanced Medical Emergency and Critical Care Center, Japanese Maebashi Red Cross Hospital, Gunma, Japan; Department of Plastic and Reconstructive Surgery, Showa University School of Medicine, Tokyo, Japan; Department of Plastic and Reconstructive Surgery, New Tokyo Hospital, Chiba, Japan; and Department of Plastic and Reconstructive Surgery, Gunma Children's Medical Center, Gunma, Japan.
Abstract:
The use of cultured epithelial autografts for the treatment of extensive burn wounds has become popular in recent years. We examined extensive burn wounds in 14 patients by using a combination of autograft and cultured epithelial autografts developed in Japan (JACE).
Methods:
We undertook a skin biopsy at 2, 4, and 6 weeks after transplantation with JACE. By using electron microscopy we observed the engraftment process.
Results:
In transmission electron microscope findings, we recognized the engraftment process of JACE. Keratinocytes matured gradually. Collagen fibers formed thick bundles in the dermis layer. In scanning electron microscope findings, we observed papillary dermis development on the artificial dermis.
Conclusions:
After managing wound bed preparation by using artificial dermis, we were able to recognize the good result of grafting JACE on meshed 6:1 split thickness autografts. This is because the auto dermis from autograft extended under the JACE, binding between JACE, and the dermis became strong.
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