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Related Concept Videos

Affinity Chromatography01:03

Affinity Chromatography

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Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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Ion-exchange chromatography, or IEC, is a technique for separating ions based on their affinity for the stationary phase. The stationary phase is a cross-linked polymer resin with covalently attached ionic functional groups. The functional groups can be either positively charged (cation exchangers) or negatively charged (anion exchangers). A cation exchanger consists of a polymeric anion and active cations, while an anion exchanger is a polymeric cation with active anions. The choice of...
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High-Performance Liquid Chromatography: Introduction01:11

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High-performance liquid chromatography(HPLC), formerly referred to as High-pressure liquid chromatography, is a powerful technique used to separate, identify, and quantify components in complex mixtures. The term "high pressure" refers to using high pressure to push the liquid mobile phase through the tightly packed columns.
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Chromatography: Introduction01:10

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Chromatography is a technique used to separate compounds based on differences of partitioning between two phases, the stationary phase and the mobile phase.
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Capillary Electrophoresis: Applications01:30

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Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
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Silica Gel Column Chromatography: Overview01:10

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Silica gel column chromatography is a technique for separating compounds using a column packed with silica gel as the stationary phase. This method relies on differences in the polarity of compounds. Based on their polarities, compounds move between the stationary phase (silica gel) and the mobile phase (the solvent), forming discrete bands in the column.
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Coupling of In Vitro Bioassays with Planar Chromatography in Effect-Directed Analysis.

Stefan C Weiss1, Nicole Egetenmeyer2, Wolfgang Schulz2

  • 1Betriebs und Forschungslaboratorium, Zweckverband Landeswasserversorgung (LW), Am Spitzigen Berg 1, 89129, Langenau, Germany. weiss.s@lw-online.de.

Advances in Biochemical Engineering/Biotechnology
|October 21, 2016
PubMed
Summary

High-performance thin-layer chromatography (HPTLC) coupled with bioassays (HPTLC-EDA) effectively identifies environmental contaminants in water. This method aids in assessing ecological risks and tracking changes in water quality over time.

Keywords:
Antibacterial effectBioautographyBioluminescence inhibitionDerivatizationEnvironmental analysisEstrogenic effectFungicidal effectHPTLC/AMDNeurotoxicityPhotosynthesis inhibitionTLC-MS

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Area of Science:

  • Environmental Chemistry
  • Analytical Chemistry
  • Ecotoxicology

Background:

  • Anthropogenic organic substances and their transformation products are increasingly detected in environmental samples.
  • The presence of these compounds poses potential risks to aquatic ecosystems.
  • Bioassays are crucial for assessing ecotoxicological risks in water samples.

Purpose of the Study:

  • To evaluate the efficacy of High-Performance Thin-Layer Chromatography-Effect-Directed Analysis (HPTLC-EDA) for identifying environmental contaminants.
  • To demonstrate the application of HPTLC-EDA in water quality assessment.
  • To highlight the advantages of HPTLC over HPLC in effect-directed analysis.

Main Methods:

  • Effect-Directed Analysis (EDA) combining physicochemical separation with in vitro bioassays.
  • High-Performance Thin-Layer Chromatography (HPTLC) for separating organic compounds.
  • Direct application of bioassays on HPTLC plates to detect biological effects.

Main Results:

  • HPTLC-EDA enables prioritization for identifying causative agents of adverse effects in complex environmental samples.
  • The method allows for tracking changes in biological activity over time, space, or processes without full substance identification.
  • Case studies on wastewater treatment and artificial turf influence on groundwater illustrate HPTLC-EDA's significance.

Conclusions:

  • HPTLC-EDA is a powerful tool for investigating water quality and identifying ecotoxicological risks.
  • The complete evaporation of the mobile phase in HPTLC offers an advantage over HPLC for bioassays.
  • HPTLC's flexibility allows coupling with various detection techniques, including mass spectrometry, for comprehensive analysis.