Related Experiment Video
Updated: Mar 13, 2026
Author Spotlight: Assessing the Potential of Circulating Tumor Cells in Leptomeningeal Disease Research
Published on: March 29, 2024
Higher positive identification of malignant CSF cells using the cytocentrifuge than the Suta chamber
Sérgio Monteiro de Almeida1,2, Indianara Rotta1,2, Arnaldo José de Conto1,2
1Universidade Federal do Paraná, Curitiba PR, Brasil.
Objective:
To define how to best handle cerebrospinal fluid (CSF) specimens to obtain the highest positivity rate for the diagnosis of malignancy, comparing two different methods of cell concentration, sedimentation and cytocentrifugation.
Methods:
A retrospective analysis of 411 CSF reports.
Results:
This is a descriptive comparative study. The positive identification of malignant CSF cells was higher using the centrifuge than that using the Suta chamber (27.8% vs. 19.0%, respectively; p = 0.038). Centrifuge positively identified higher numbers of malignant cells in samples with a normal concentration of white blood cells (WBCs) (< 5 cells/mm3) and with more than 200 cells/mm3, although this was not statistically significant. There was no lymphocyte loss using either method.
Conclusions:
Cytocentrifugation positively identified a greater number of malignant cells in the CSF than cytosedimentation with the Suta chamber. However, there was no difference between the methods when the WBC counts were within the normal range.
