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Related Experiment Videos

Changes in endoplasmic reticulum during spermiogenesis in the mouse.

T Nakamoto1, Y Sakai

  • 1Department of Anatomy, School of Medicine, Kitasato University, Kanagawa, Japan.

Cell and Tissue Research
|August 1, 1989
PubMed
Summary

The endoplasmic reticulum in mouse spermatids transforms into a radial body during spermiogenesis. This organelle network aggregates, condenses, and disappears, suggesting a coordinated removal process.

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Area of Science:

  • Cell Biology
  • Reproductive Biology
  • Microscopy

Background:

  • Spermiogenesis involves significant cellular reorganization.
  • The role of the endoplasmic reticulum during spermatid maturation is not fully understood.
  • Three-dimensional visualization techniques are crucial for studying complex cellular structures.

Purpose of the Study:

  • To investigate the dynamic changes of the endoplasmic reticulum in mouse spermatids during spermiogenesis.
  • To elucidate the three-dimensional structure and transformation of the endoplasmic reticulum.
  • To understand the relationship between different endoplasmic reticulum structures and their disappearance.

Main Methods:

  • Scanning electron microscopy with the OsO4-DMSO-OsO4 method for 3D organelle observation.

Related Experiment Videos

  • Transmission electron microscopy of Ur-Pb-Cu stained sections for detailed ultrastructure.
  • Analysis of endoplasmic reticulum morphology in early and late maturation phase spermatids.

    • Main Results:

    • The endoplasmic reticulum forms a complex 3D network in early spermatids.
    • A 2D endoplasmic reticulum network is observed beneath the plasma membrane and around Sertoli cell processes.
    • During maturation, the endoplasmic reticulum aggregates into a 'radial body' and subsequently disappears.

    Conclusions:

    • The endoplasmic reticulum undergoes significant morphological changes during spermiogenesis.
    • The formation of the radial body represents a condensation and transformation of the endoplasmic reticulum.
    • Both 2D and 3D endoplasmic reticulum networks disappear concurrently, suggesting a related mechanism.