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Analysis of Histone Deacetylases Sumoylation by Immunoprecipitation Techniques
Tobias Wagner1, Maren Godmann1, Thorsten Heinzel2
1Department of Biochemistry, Institute of Biochemistry and Biophysics, CMB - Center for Molecular Biomedicine, Friedrich Schiller University Jena, Hans-Knöll-Str. 2, Jena, 07745, Germany.
Abstract:
Histone deacetylases (HDACs) are controlling dynamic protein acetylation by removing acetyl moieties from lysine. Histone deacetylases themselves are regulated on the posttranslational level, including modifications with small ubiquitin-like modifier (SUMO) proteins. Detecting SUMO modifications of deacetylases by immunoblotting is technically challenging due to the typically low ratio of the modified compared to the unmodified species. Here, we describe a set of methods for the detection of endogenous sumoylated HDACs by immunoprecipitation and immunoblotting techniques.
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