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Model System-Guided Protein Interaction Mapping for Virus Isolated from Phloem Tissue.

Stacy L DeBlasio1,2, Richard S Johnson3, Michael J MacCoss3

  • 1Agricultural Research Service, USDA , Ithaca, New York 14853, United States.

Journal of Proteome Research
|October 21, 2016
PubMed
Summary

This study identified Potato leafroll virus (PLRV) host protein interactions in infected potato plants. Proteomics reveals key proteins involved in viral infection, validating model systems for studying phloem-limited viruses.

Keywords:
LuteoviridaePotato leafroll virusSAINTSolanum tuberosumco-immunoprecipitationhigh-resolution mass spectrometryhost−pathogen interactionsmodel systemphloem-limitedpolerovirus

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Area of Science:

  • Plant Pathology
  • Molecular Biology
  • Proteomics

Background:

  • Phloem-limited plant viruses are difficult to study due to their location.
  • Understanding host-virus interactions is crucial for disease management.

Purpose of the Study:

  • To identify Potato leafroll virus (PLRV)-host protein complexes in its natural host, potato.
  • To assess the utility of model systems for studying phloem-limited viruses.

Main Methods:

  • Isolation of PLRV-host protein complexes from systemically infected potato using co-immunoprecipitation (co-IP) and mass spectrometry (MS).
  • Comparison of two co-IP support matrices.
  • Bioinformatic analysis of identified protein interactions.

Main Results:

  • Identification of 44 potato proteins and the viral P1 protein specifically associated with PLRV in infected phloem.
  • An additional 142 proteins were found to interact with PLRV, with over 80% previously identified in model hosts.
  • Enrichment analysis revealed proteins involved in plasmodesmata, organelle transport, translation, and mRNA processing.

Conclusions:

  • Model system proteomics experiments are highly valuable for studying protein interactions of phloem-limited viruses.
  • This research provides insights into the molecular mechanisms of PLRV infection in its natural host.