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Related Experiment Videos

Measuring tissue factor (factor III) activity in plasma.

C Fukuda1, K Iijima, K Nakamura

  • 1Department of Medical Technology, Tottori University College of Medical Care Technology, Yonago, Japan.

Clinical Chemistry
|September 1, 1989
PubMed
Summary
This summary is machine-generated.

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This study presents a new method to measure tissue factor (TF) activity in plasma using a chromogenic substrate. The technique effectively quantifies low TF levels, crucial for diagnosing conditions like disseminated intravascular coagulation.

Area of Science:

  • Biochemistry
  • Hematology
  • Clinical Diagnostics

Background:

  • Tissue factor (TF) plays a critical role in hemostasis and thrombosis.
  • Accurate measurement of TF activity in plasma is essential for diagnosing coagulation disorders.
  • Existing methods may struggle to quantify low levels of TF activity accurately.

Purpose of the Study:

  • To develop and validate a novel chromogenic assay for quantifying plasma tissue factor (TF) activity.
  • To improve the sensitivity of TF measurement, enabling detection of low activity levels.
  • To assess the utility of the assay in identifying TF activity associated with specific clinical conditions.

Main Methods:

  • Plasma pretreatment involved preparing the euglobulin fraction to remove inhibitors.

Related Experiment Videos

  • Heating the euglobulin fraction at 60°C for 3 minutes removed fibrinogen.
  • A chromogenic substrate was used to quantify TF activity, with measurements taken in arbitrary units/L.

    • Main Results:

    • The developed method demonstrated good within-run and day-to-day reproducibility.
    • The assay successfully measured low TF activity levels that were previously undetectable.
    • Normal plasma TF activity was determined to be 1.02 (SD 0.91) arbitrary units/L.
    • Significantly elevated TF activity (≥20 arbitrary units/L) was observed in patients with disseminated intravascular coagulation.

    Conclusions:

    • This novel chromogenic assay provides a reliable and sensitive method for measuring plasma TF activity.
    • The assay's ability to detect low TF levels aids in the diagnosis of coagulation disorders.
    • Elevated TF activity is a significant indicator in patients with disseminated intravascular coagulation, highlighting the assay's clinical relevance.