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Green-to-red primed conversion of Dendra2 using blue and red lasers
N V Klementieva1, K A Lukyanov, N M Markina
1Nizhny Novgorod State Medical Academy, Nizhny Novgorod, Russia.
Chemical Communications (Cambridge, England)
|November 2, 2016
Summary
Primed conversion of fluorescent protein Dendra2 can now be induced using common red lasers, expanding its use in microscopy. This discovery simplifies the process for advanced biological imaging applications.
Area of Science:
- Biophysics
- Optical Microscopy
- Fluorescent Proteins
Background:
- Fluorescent proteins like Dendra2 are crucial for live-cell imaging.
- Primed conversion of Dendra2 typically requires specific blue and near-infrared laser combinations.
- This process has limitations in accessibility and compatibility with standard microscopy setups.
Purpose of the Study:
- To investigate alternative laser wavelengths for inducing Dendra2 primed conversion.
- To determine if commonly available red lasers can trigger this phenomenon.
- To enhance the accessibility of Dendra2 photoconversion for broader research applications.
Main Methods:
- Utilized red lasers (630-650 nm) for excitation of Dendra2.
- Performed experiments on Dendra2-expressing samples.
- Analyzed the conversion efficiency and characteristics using microscopy techniques.
Main Results:
- Demonstrated successful primed conversion of Dendra2 using red lasers (630-650 nm).
- Confirmed that red laser excitation is sufficient to induce the primed conversion phenomenon.
- Showcased the potential for using standard confocal and single-molecule detection microscopes.
Conclusions:
- Red lasers provide a more accessible method for Dendra2 primed conversion.
- This finding broadens the applicability of Dendra2 in various microscopy techniques.
- Simplifies experimental workflows for researchers using photoconvertible fluorescent proteins.
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