Related Experiment Videos
In vitro susceptibility of Pseudomonas mallei to antimicrobial agents
1Department of Medicine, College of Veterinary Medicine, Al-America, Baghdad, Iraq.
Abstract:
Pseudomonas mallei was isolated from pus samples obtained from 34 mallein-positive horses. The isolates were subjected to in vitro sensitivity test using 16 different antimicrobial discs. All isolates (34) were sensitive to sulfamethizole, gentamycin, tetracycline, sulfathiazole, kanamycin, tobramycin, streptomycin and a combination of trimethoprim and sulfamethoxazole while none of them were sensitive to cephalothin, colistin, ampicillin, penicillin and nitrofurantoin. Rifapicin, chloramphenicol and carbenicillin were effective against 32, 26 and 18 isolates respectively. The minimum inhibitory concentrations (MICs) of gentamycin, tetracycline, tobramycin, sulfamethizole, streptomycin, rifampicin and a combination of trimethoprim and sulfamethoxazole were 0.28, 0.38, 0.67, 1.40, 3.40, 5.86 and 5.30 micrograms/ml, respectively.
Insights
This study tested 34 Pseudomonas mallei horse isolates against 16 antimicrobials. Sulfamethizole, gentamycin, and tetracycline showed high effectiveness, while cephalothin and penicillin were ineffective.
Area of Science:
- Veterinary Microbiology
- Antimicrobial Resistance
Background:
- Pseudomonas mallei causes significant disease in horses.
- Understanding antimicrobial susceptibility is crucial for effective treatment strategies.
Purpose of the Study:
- To determine the in vitro antimicrobial susceptibility profile of Pseudomonas mallei isolates from horses.
- To identify effective antimicrobial agents for treating P. mallei infections.
Main Methods:
- Isolation of Pseudomonas mallei from pus samples of mallein-positive horses.
- In vitro antimicrobial sensitivity testing using 16 different antimicrobial discs.
- Determination of Minimum Inhibitory Concentrations (MICs) for selected effective agents.
Main Results:
- All 34 isolates were sensitive to sulfamethizole, gentamycin, tetracycline, sulfathiazole, kanamycin, tobramycin, streptomycin, and trimethoprim/sulfamethoxazole.
- No isolates were sensitive to cephalothin, colistin, ampicillin, penicillin, or nitrofurantoin.
- Rifampicin, chloramphenicol, and carbenicillin showed varying degrees of efficacy.
Conclusions:
- Several antimicrobial agents, including gentamycin, tetracycline, and sulfamethizole, are effective against Pseudomonas mallei in vitro.
- Certain antibiotics like penicillin and cephalothin are not recommended for treating P. mallei infections based on these findings.