omega-Hydroxyacid derivatives in the epidermis of several mammalian species

1. omega-Hydroxyacids from the acylceramides and acylglucosylceramides of mammalian epidermis were examined by thin-layer and gas-liquid chromatography to determine their degree of unsaturation and chain-length distributions. The species examined included human (Homo sapiens), pig (Sus scrofa), mouse (Mus musculus) and rat (Rattus rattus). 2. Within a species, the omega-hydroxyacids from the acylceramide and acylglucosylceramide were essentially identical. 3. The human omega-hydroxyacids proved to be mainly saturated with C30:0 being the major entity. 4. The pig contained similar saturated, monoenoic and small amounts of dienoic omega-hydroxyacids, with C30:0, C32:1 being the major entities. 5. The mouse and rat contained C32:0 and C34:1 as the major components.