Insecticidal Constituents from Buddlej aalbiflora Hemsl.

Area of Science:

• Natural Product Chemistry
• Entomology
• Agricultural Science

Background:

• Buddleja albiflora Hemsl is a plant species with potential medicinal properties.
• Insect pests like Plutella xylostella and Macrosiphoniella sanborni cause significant agricultural damage.
• Identifying natural compounds with insecticidal activity is crucial for sustainable pest management.

Purpose of the Study:

• To isolate and identify known compounds from Buddleja albiflora Hemsl.
• To evaluate the insecticidal activity of these isolated compounds against key agricultural pests.
• To determine the efficacy of these compounds as potential bio-insecticides.

Main Methods:

• Phytochemical analysis of MeOH extract of Buddleja albiflora Hemsl.
• Isolation and identification of eleven known compounds: deoxymikanolide, 1,3-dihydroxyxanthone, kumatakenin, apigenin, chrysin, kaempferol, Iso-kaempferol, luteolin, luteolin-3',4'-dimethylether-7-O-β-glucoside, luteolin-7-O-β-glucoside, and quercetin.
• Bioassays were conducted to determine insecticidal activity against larvae of Plutella xylostella, Mythimna separata, and Macrosiphoniella sanborni.

Main Results:

• Eleven known compounds were successfully identified from the plant extract.
• The compounds exhibited varying degrees of insecticidal activity.
• The lowest 50% anti-feedant concentration (AFC50) against P. xylostella was 0.0058 mg L-1.
• The lowest 50% lethal concentration (LC50) against P. xylostella and M. sanborni were 0.0046 mg L-1 and 3.4048 mg L-1, respectively.

Conclusions:

• Buddleja albiflora Hemsl contains compounds with significant insecticidal potential.
• Deoxymikanolide and other identified compounds demonstrate efficacy against Plutella xylostella and Macrosiphoniella sanborni.
• These findings suggest that Buddleja albiflora Hemsl can be a valuable source for developing natural insecticides.