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Long Non-coding RNA TUSC7, a Target of miR-23b, Plays Tumor-Suppressing Roles in Human Gliomas

Chao Shang1, Yan Guo2, Yang Hong3

  • 1Department of Neurobiology, College of Basic Medical Sciences, China Medical University Shenyang, China.

Frontiers in Cellular Neuroscience
|October 22, 2016
PubMed

Insights

Tumour suppressor candidate 7 (TUSC7) acts as a tumor suppressor in glioma by inhibiting cell proliferation and invasion. This long non-coding RNA (lncRNA) is downregulated in glioma and suggests a new therapeutic strategy.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The role of the novel tumor suppressor gene, Tumour suppressor candidate 7 (TUSC7), and its long non-coding RNA (lncRNA) in human brain glioma remains unclear.
  • Glioma is a significant cause of cancer-related mortality, necessitating research into novel therapeutic targets and biomarkers.

Purpose of the Study:

  • To investigate the expression and function of TUSC7 in human brain glioma.
  • To determine the relationship between TUSC7 and miR-23b in glioma.
  • To evaluate TUSC7 as a prognostic biomarker for glioma patients.

Main Methods:

  • Quantitative real-time PCR to assess TUSC7 and miR-23b expression in glioma tissues and cell lines.
  • Bioinformatics analysis to predict interactions between TUSC7 and microRNAs.
  • Cell proliferation, apoptosis, and invasion assays to determine TUSC7 function.
  • RNA pull-down and luciferase reporter assays to validate TUSC7-miR-23b interaction.

Main Results:

  • TUSC7 expression was significantly lower in glioma tissues and cell lines compared to normal controls, correlating with higher histological grade.
  • TUSC7 upregulation suppressed glioma cell proliferation and invasion while promoting apoptosis.
  • TUSC7 directly binds to miR-23b, and miR-23b is upregulated in glioma, showing a negative correlation with TUSC7 expression.
  • TUSC7 inhibited glioma cell progression largely independent of miR-23b.

Conclusions:

  • TUSC7 functions as a tumor suppressor lncRNA in glioma, negatively regulated by miR-23b.
  • TUSC7 serves as a potential prognostic biomarker for glioma.
  • Targeting TUSC7 represents a promising novel therapeutic strategy for glioma treatment.
Keywords:
TUSC7gliomamir-23bnoncoding RNAtumor suppress gene

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