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Updated: Mar 13, 2026

Generation of Fluorescent Protein Fusions in Candida Species
Published on: March 4, 2017
Genetic Transformation of Candida glabrata by Heat Shock
Fabian Istel1, Tobias Schwarzmüller1, Michael Tscherner1
1Medical University Vienna, Max F. Perutz Laboratories, Department of Medical Biochemistry, Vienna, Austria.
Abstract:
Here, we report a method for the transformation of Candida glabrata using a heat shock method. The protocol can be used for transformations in single well or in 96-well scale. It has been employed as an alternative method to the electroporation protocol to construct a genome-scale gene deletion collection in the human fungal pathogen Candida glabrata ATCC2001 and related strains. Furthermore, the protocol can be used to generate gene deletions in clinical isolates of Candida glabrata (C. glabrata).
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