Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Direct Counting of mRNA Copies Inside Individual Lipid Nanoparticles Using In Situ Lysis and Labeling.

Analytical chemistry·2026
Same author

New frontiers in applied biophysics: advancing drug discovery using single-molecule microscopy.

Biophysical reviews·2026
Same author

A guide RNA repeat checkpoint steers CRISPR-Cas9 catalysis.

bioRxiv : the preprint server for biology·2026
Same author

Microtubules: Decoding tubulin diversity with help from an amoeba.

Current biology : CB·2025
Same author

Preparation of Polarity-Marked Microtubules Using a Plus-End Capping DARPin.

Bio-protocol·2024
Same author

The minus-end depolymerase KIF2A drives flux-like treadmilling of γTuRC-uncapped microtubules.

The Journal of cell biology·2023

Related Experiment Video

Updated: Mar 13, 2026

Hand Controlled Manipulation of Single Molecules via a Scanning Probe Microscope with a 3D Virtual Reality Interface
11:00

Hand Controlled Manipulation of Single Molecules via a Scanning Probe Microscope with a 3D Virtual Reality Interface

Published on: October 2, 2016

9.6K

Manipulating and Visualizing Molecular Interactions in Customized Nanoscale Spaces.

Gil Henkin1, Daniel Berard1, Francis Stabile1

  • 1Department of Physics, McGill University , Montreal, Quebec, Canada H3A 2T8.

Analytical Chemistry
|November 4, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a dynamic nanofluidic platform to precisely control and visualize biomolecule interactions and reactions in real-time. The advanced system offers new insights into DNA molecule behavior under varying chemical conditions.

More Related Videos

Novel 3D/VR Interactive Environment for MD Simulations, Visualization and Analysis
11:29

Novel 3D/VR Interactive Environment for MD Simulations, Visualization and Analysis

Published on: December 18, 2014

12.3K
Scalable Nanohelices for Predictive Studies and Enhanced 3D Visualization
08:03

Scalable Nanohelices for Predictive Studies and Enhanced 3D Visualization

Published on: November 12, 2014

11.0K

Related Experiment Videos

Last Updated: Mar 13, 2026

Hand Controlled Manipulation of Single Molecules via a Scanning Probe Microscope with a 3D Virtual Reality Interface
11:00

Hand Controlled Manipulation of Single Molecules via a Scanning Probe Microscope with a 3D Virtual Reality Interface

Published on: October 2, 2016

9.6K
Novel 3D/VR Interactive Environment for MD Simulations, Visualization and Analysis
11:29

Novel 3D/VR Interactive Environment for MD Simulations, Visualization and Analysis

Published on: December 18, 2014

12.3K
Scalable Nanohelices for Predictive Studies and Enhanced 3D Visualization
08:03

Scalable Nanohelices for Predictive Studies and Enhanced 3D Visualization

Published on: November 12, 2014

11.0K

Area of Science:

  • Nanofluidics
  • Biomolecular Interactions
  • Single-Molecule Biophysics

Background:

  • Convex lens-induced confinement (CLiC) enables imaging molecules under confinement.
  • Existing methods lack in situ control over the chemical environment during nanoscale imaging.
  • Understanding dynamic biomolecular processes requires advanced visualization and kinetic analysis tools.

Purpose of the Study:

  • To develop a dynamically adjustable nanofluidic platform for formatting biomolecule conformations.
  • To visualize and quantify interaction kinetics between biomolecules in solution with enhanced time resolution.
  • To study salt-, surfactant-, and enzyme-induced reactions involving DNA molecules under controlled nanoscale confinement.

Main Methods:

  • Extension of the CLiC technique with an in situ chemical environment modification system.
  • Utilizing a deep microchannel for diffusive reagent exchange within a nanoscale imaging region.
  • Employing a nanopost array to fix the imaging region height and confining nanogrooves for molecule formatting.
  • Time-resolved single-molecule measurements of DNA conformational changes and reactions.

Main Results:

  • Observed two distinct salt-induced transitions in DNA molecules (polymer extension and dye release) on separate time scales.
  • Quantified single-molecule DNA compaction trajectories induced by cationic surfactants under confinement.
  • Directly visualized enzyme-induced DNA cleavage at multiple sites within the adjustable nanoscale confinement.
  • Demonstrated the platform's capability to provide kinetic information on dynamic chemical processes at the single-molecule level.

Conclusions:

  • The developed nanofluidic platform offers unprecedented control and time resolution for studying biomolecular reactions.
  • The system provides novel insights into DNA molecule behavior, including salt-induced transitions and surfactant-mediated compaction.
  • This technology facilitates quantitative exploration of physiologically and biotechnologically relevant nanoscale processes.