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Related Concept Videos

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Related Experiment Video

Updated: Mar 13, 2026

Whole-cell Super-Resolution Imaging via DNA-PAINT on a Spinning Disk Confocal with Optical Photon Reassignment
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Super-Resolution Real Imaging in Microsphere-Assisted Microscopy.

Hok Sum Sam Lai1, Feifei Wang2,3, Yi Li1

  • 1Mechanical and Biomedical Engineering, City University of Hong Kong, Tat Chee Avenue, Hong Kong.

Plos One
|October 22, 2016
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Summary

High-refractive index microspheres enable real super-resolution imaging, surpassing previous virtual image limitations. This technique magnifies sub-diffraction-limit features up to 10x, advancing optical microscopy capabilities.

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Area of Science:

  • Optics and Photonics
  • Microscopy
  • Nanotechnology

Background:

  • Microsphere-assisted microscopy offers simplicity and diffraction-limit surpassing capabilities.
  • Previous methods only achieved sub-diffraction imaging via virtual images.
  • Real imaging of sub-diffraction features remained a challenge.

Purpose of the Study:

  • To demonstrate real super-resolution imaging using high-refractive index microspheres.
  • To investigate the impact of microsphere properties (refractive index, size) on image formation.
  • To explore the relationship between focus position and magnification for real imaging.

Main Methods:

  • Utilized high-refractive index microspheres for imaging.
  • Experimentally and theoretically investigated microsphere parameters and focus positions.
  • Analyzed image magnification and field-of-view characteristics.

Main Results:

  • Successfully formed real, super-resolution images using microspheres.
  • Observed that microsphere refractive index and size significantly affect image planes and formation.
  • Demonstrated up to 10x magnification of sub-diffraction features with a 9 μm field-of-view.

Conclusions:

  • High-refractive index microspheres enable practical real super-resolution imaging.
  • Microsphere properties can be tuned to optimize imaging parameters.
  • This method enhances the capabilities of optical microscopy for nanoscale visualization.