Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chromatin Packaging01:32

Chromatin Packaging

20.1K
Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
20.1K
Chromatin Packaging02:21

Chromatin Packaging

22.8K
Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
The chromatin
In combination with specialized DNA binding protein called Histones, the DNA double helix forms a compact DNA: protein complex called chromatin. The chromatin itself is further compacted into higher-order...
22.8K
Genomic DNA in Eukaryotes00:58

Genomic DNA in Eukaryotes

53.7K
Eukaryotes have large genomes compared to prokaryotes. To fit their genomes into a cell, eukaryotic DNA is packaged extraordinarily tightly inside the nucleus. To achieve this, DNA is tightly wound around proteins called histones, which are packaged into nucleosomes that are joined by linker DNA and coil into chromatin fibers. Additional fibrous proteins further compact the chromatin, which is recognizable as chromosomes during certain phases of cell division.
53.7K
The Nucleosome02:33

The Nucleosome

19.6K
DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
19.6K
The Nucleosome01:19

The Nucleosome

4.6K
Human DNA is almost two meters long. However, it is compressed inside a tiny nucleus measuring only a few microns in diameter. To make this degree of compaction possible, DNA is organized into several sequential levels so that it can fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
In a chromosome, DNA is wound twice around a protein complex called a histone octamer core, which consists of 8 histone proteins. This...
4.6K
The Nucleosome02:33

The Nucleosome

5.5K
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

ARID1A Mediates SWI/SNF-Independent Maintenance of Heterochromatin Architecture to Restrain Viral Mimicry and Immunogenicity in Colon Cancer.

Cancer research·2026
Same author

A paracrine-to-autocrine shunt of GREM1 fuels colorectal cancer metastasis via ACVR1C.

Molecular cancer·2026
Same author

Cell-free DNA end characteristics enable accurate and sensitive cancer diagnosis.

Cell reports methods·2024
Same author

Nuclear Smooth Muscle α-actin Participates in Vascular Smooth Muscle Cell Differentiation.

Nature cardiovascular research·2024
Same author

Natural compounds: Wnt pathway inhibitors with therapeutic potential in lung cancer.

Frontiers in pharmacology·2023
Same author

Nuclear Smooth Muscle α-actin in Vascular Smooth Muscle Cell Differentiation.

Research square·2023

Related Experiment Video

Updated: Mar 13, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
10:40

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

23.2K

Stringing Nucleosome Necklaces in the Yeast Genome.

Prabodh Kapoor1, Xuetong Shen2

  • 1Department of Cellular and Molecular Biology, The University of Texas Health Science Center, Tyler, TX 75708-3154, USA.

Cell
|October 22, 2016
PubMed
Summary

Researchers identified key protein factors that directly influence nucleosome positioning in yeast. They broke down this complex process into four distinct, sequential stages for better understanding.

More Related Videos

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol
10:33

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

Published on: November 17, 2023

1.8K
Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.7K

Related Experiment Videos

Last Updated: Mar 13, 2026

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
10:40

Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA

Published on: September 10, 2013

23.2K
Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol
10:33

Author Spotlight: Advancing Chromatin Research and Overcoming Limitations with a High-Enrichment Locus-Specific Chromatin Isolation Protocol

Published on: November 17, 2023

1.8K
Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.7K

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Nucleosome positioning is crucial for regulating DNA accessibility and gene expression.
  • Understanding the factors that govern nucleosome positioning is essential for deciphering genome regulation.

Purpose of the Study:

  • To investigate the direct roles of specific proteins in yeast nucleosome positioning.
  • To develop a conceptual framework for the stages involved in nucleosome positioning.

Main Methods:

  • Utilized a reconstituted system with yeast genomic DNA and purified proteins.
  • Performed biochemical assays to observe protein-DNA interactions and nucleosome formation.

Main Results:

  • Identified key protein factors that directly contribute to the precise positioning of nucleosomes.
  • Demonstrated the sequential nature of nucleosome positioning mediated by these factors.
  • Conceptualized the nucleosome positioning process into four distinct stages.

Conclusions:

  • The study elucidates the direct mechanistic contributions of specific proteins to nucleosome positioning.
  • A four-stage model provides a new framework for understanding yeast chromatin organization and regulation.