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Acetamiprid multidetection by disposable electrochemical DNA aptasensor.

R Rapini1, A Cincinelli1, G Marrazza1

  • 1Department of Chemistry "Ugo Schiff", University of Florence, Via della Lastruccia 3, Sesto Fiorentino (FI) 50019, Italy.

Talanta
|October 23, 2016
PubMed
Summary
This summary is machine-generated.

This study introduces a novel electrochemical DNA aptasensor for detecting acetamiprid pesticide. The developed sensor offers sensitive detection, showing promise for real-world applications in food safety.

Keywords:
AcetamipridAptamerElectrochemical sensorScreen-printed electrode

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Area of Science:

  • Electrochemistry
  • Biosensors
  • Analytical Chemistry

Background:

  • Pesticide detection is crucial for food safety and environmental monitoring.
  • Existing methods for acetamiprid detection can be complex and time-consuming.
  • Development of sensitive and rapid detection platforms is needed.

Purpose of the Study:

  • To develop a sensitive electrochemical DNA aptasensor for acetamiprid detection.
  • To utilize a competitive assay format with disposable screen-printed arrays.
  • To enhance sensor performance through material modification and optimized assay conditions.

Main Methods:

  • Fabrication of a modified graphite screen-printed electrode using polyaniline and gold nanoparticles.
  • Immobilization of DNA aptamers on gold nanoparticles for acetamiprid recognition.
  • Employing a competitive assay format with enzymatic signal amplification (streptavidin-alkaline phosphatase).
  • Detection of the enzymatic product via differential pulse voltammetry.

Main Results:

  • The aptasensor demonstrated a signal-off response to increasing acetamiprid concentrations.
  • A linear dose-response was observed in the range of 0.25–2.0 µM acetamiprid.
  • A limit of detection (LOD) of 0.086 µM was achieved under optimized conditions.
  • The aptasensor showed selectivity, confirmed by atrazine analysis, and was tested in spiked fruit juice samples.

Conclusions:

  • The developed electrochemical DNA aptasensor provides a sensitive and selective method for acetamiprid detection.
  • The use of disposable screen-printed arrays and a competitive format offers a practical approach for on-site monitoring.
  • This aptasensor shows potential for the rapid and reliable analysis of pesticides in food samples.