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Updated: Mar 13, 2026

CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells

CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells

Published on: October 18, 2014

A microchip for integrated single-cell genotoxicity assay.

Hui Dong¹, Hao Sun¹, Jianping Zheng²

¹School of Mechanical Engineering and Automation, Fuzhou University, Fujian 350116, China.

|October 23, 2016

Summary

This summary is machine-generated.

This study introduces a microchip for single-cell gene expression profiling in breast cancer. It enables precise analysis of drug-induced genotoxicity, reducing reagent use and costs.

Keywords:

Genotoxicity assay Integrated microfluidics Single-cell multiplex qPCR

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Last Updated: Mar 13, 2026

CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells

CometChip: A High-throughput 96-Well Platform for Measuring DNA Damage in Microarrayed Human Cells

Published on: October 18, 2014

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

The Lambda Select cII Mutation Detection System

The Lambda Select cII Mutation Detection System

Published on: April 26, 2018

Area of Science:

Biomedical Engineering
Molecular Biology
Cancer Research

Background:

Precision medicine and large-scale biologic databases are advancing biomedical research.
Cancer is understood as a disease linked to genetic variations and genomic damage.
Single-cell analysis using microfluidics offers potential for improved cancer risk assessment, diagnosis, and treatment.

Purpose of the Study:

To develop and present a multi-layer microchip for single-cell gene expression profiling.
To enable the precision study of drug-induced genotoxicity in cancer cells.

Main Methods:

Human breast cancer cells (MCF-7) were treated with varying concentrations and durations of methyl methanesulfonate, docetaxel, and colchicine.
On-chip cell lysis, mRNA capture, and reverse transcription to cDNA on microbeads were performed.
Triplex real-time polymerase chain reactions (qPCR) were used for simultaneous amplification and quantification of GAPDH, CDKN1A, and AURKA genes.

Main Results:

The developed microchip successfully integrated all steps for single-cell gene expression profiling.
The system demonstrated the capability for precision study of drug-induced genotoxicity.
Reduced reagent consumption per reaction and lower instrumental costs were achieved.

Conclusions:

The multi-layer microchip is an effective tool for single-cell gene expression profiling.
This technology facilitates precise investigation of genotoxicity in cancer cells.
The platform offers a cost-effective and reagent-efficient approach for cancer research and drug development.