Related Experiment Video
Updated: Aug 1, 2026

16:24
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells
Published on: February 21, 2014
Mitogenic effect of estradiol on MCF-7 human breast cancer cells can be modulated by serum
1Laboratoire de Biochimie, Faculté de Pharmacie, Université de Limoges, France.
Abstract:
The MCF-7 human breast cancer cell line responds to estradiol stimulation in vitro by increased proliferation only if prolonged subcultures in dextran-coated charcoal-treated fetal calf serum have been made previously. This growth stimulation is not obtained when cells are grown in medium containing 5% untreated fetal calf serum. We describe here the culture conditions under which we obtain a reproducible estradiol effect on cell growth.

