Immobilized aptamer paper spray ionization source for ion mobility spectrometry

Area of Science:

• Analytical Chemistry
• Biomedical Analysis
• Separation Science

Background:

• Traditional sample preparation for ion mobility spectrometry (IMS) can be complex and time-consuming.
• Integrating sample preparation with ionization is crucial for streamlining analytical workflows.

Purpose of the Study:

• To develop a novel paper spray ionization source integrated with selective microextraction for IMS.
• To demonstrate the feasibility of using aptamer-immobilized paper for simultaneous extraction and ionization of analytes.

Main Methods:

• A thin-film microextraction technique using aptamer immobilized on cellulose paper was employed as a paper spray ionization source for IMS.
• A custom sample introduction system facilitated continuous solvent flow for simultaneous elution and spraying.
• Optimization of experimental parameters including voltage, solvent type, and flow rate was performed.

Main Results:

• The method successfully integrated sample preparation and analyte ionization onto a single Whatman paper substrate.
• Validation demonstrated promising sensitivity and reproducibility for analyzing codeine and acetamiprid.
• The immobilized aptamer paper-spray approach proved effective for direct analysis in IMS.

Conclusions:

• The developed method offers a simplified and efficient approach for direct analysis in IMS.
• Aptamer-immobilized paper spray ionization is a viable technique for selective extraction and ionization in biomedical applications.