Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biofilms01:29

Biofilms

1.7K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Detection of submicron titanium debris associated with peri-implantitis.

Discover nano·2026
Same author

Response of murine fibroblasts or human keratinocytes to micro- and nano-scale titanium particles: the permeability of particles across keratinocytes' monolayers.

Clinical oral investigations·2025
Same author

Toxicity of resin-matrix composites in a dental clinical setting.

Odontology·2025
Same author

Magnetic Ionic Liquid: A Multifunctional Platform for the Design of Hybrid Graphene/Carbon Nanotube Networks as Electromagnetic Wave-Absorbing Materials.

Molecules (Basel, Switzerland)·2025
Same author

Electrospun Poly(vinylidene fluoride) Nanocomposites with Ionic Liquid Functionalized Graphene Nanoplatelets by a Noncovalent Method for Piezoresistive Pressure Sensor Applications.

ACS omega·2024
Same author

The Fatigue Behavior of Narrow Single-Body Titanium Implants in Saline and Acidic Media.

The International journal of oral & maxillofacial implants·2024

Related Experiment Video

Updated: Mar 13, 2026

Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices
10:57

Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices

Published on: April 22, 2022

9.3K

Biofilm behavior on sulfonated poly(ether-ether-ketone) (sPEEK).

Juan F D Montero1, Henrique A Tajiri2, Guilherme M O Barra2

  • 1Center for Research on Dental Implants (CEPID), School of Dentistry (ODT), Federal University of Santa Catarina (UFSC), Florianópolis, SC 88040-900, Brazil.

Materials Science & Engineering. C, Materials for Biological Applications
|October 25, 2016
PubMed
Summary

Sulfonated poly(ether-ether-ketone) (sPEEK) with a sulfonation degree achieved between 2 and 3 hours significantly reduces biofilm growth. This modification offers a promising strategy to prevent infections in biomedical applications.

Keywords:
BiofilmEnterococcus faecalisPEEKStreptococcus mutanssPEEK

More Related Videos

Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment
04:51

Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment

Published on: March 1, 2024

1.6K
Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
10:17

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

Published on: October 9, 2016

16.4K

Related Experiment Videos

Last Updated: Mar 13, 2026

Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices
10:57

Generation of Greater Bacterial Biofilm Biomass using PCR-Plate Deep Well Microplate Devices

Published on: April 22, 2022

9.3K
Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment
04:51

Author Spotlight: Characterizing Environmental Biofilm Mechanics Using Optical Coherence Elastography and its Applications in Wastewater Treatment

Published on: March 1, 2024

1.6K
Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors
10:17

Methodologies for Studying B. subtilis Biofilms as a Model for Characterizing Small Molecule Biofilm Inhibitors

Published on: October 9, 2016

16.4K

Area of Science:

  • Biomaterials Science
  • Surface Chemistry
  • Microbiology

Background:

  • Poly(ether-ether-ketone) (PEEK) is a versatile biomaterial.
  • Incorporating therapeutic compounds into PEEK is desirable for biomedical applications.
  • Sulfonation modifies PEEK's structure, creating sulfonated-PEEK (sPEEK).

Purpose of the Study:

  • To evaluate the impact of varying sulfonation degrees of sPEEK on biofilm formation.
  • To assess the anti-biofilm efficacy of sPEEK against Streptococcus mutans and Enterococcus faecalis.
  • To explore sPEEK as a strategy for infection prevention in biomedical implants.

Main Methods:

  • PEEK functionalization using sulfuric acid and dissolution in dimethyl-sulfoxide.
  • sPEEK thin film synthesis via dip coating.
  • Characterization of sulfonation degree (FT-IR, H NMR, TG, IEC) and surface properties (SEM, profilometry, contact angle).
  • Biofilm quantification using spectrophotometry, colony-forming units (CFUmL⁻¹), and SEM.

Main Results:

  • Thermal and chemical analyses confirmed increased sulfonation degree at 2 and 2.5 hours.
  • Statistically significant differences (p<0.05) in biofilm growth were observed between 2 and 3-hour sulfonation groups.
  • A significant reduction (p<0.05) in CFUmL⁻¹ for S. mutans and E. faecalis biofilms was recorded on sPEEK sulfonated for 2.5 and 3 hours.

Conclusions:

  • Sulfonation of PEEK for 2 to 3 hours effectively reduces Streptococcus mutans and Enterococcus faecalis biofilm growth.
  • sPEEK demonstrates potential as an anti-biofilm material for biomedical applications.
  • This approach offers an alternative strategy to embed therapeutic compounds into PEEK, mitigating implant-associated infections.