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Characterization of a novel blaIMP gene, blaIMP-58, using whole genome sequencing in a Pseudomonas putida isolate
Dennis Back Holmgaard1, Frank Hansen2, Henrik Hasman2
1Department of Clinical Microbiology, Rigshospitalet, Copenhagen, Denmark.
Abstract:
A multidrug-resistant strain of Pseudomonas putida was isolated from the urine of a 65-year-old women hospitalized for serious clinical conditions. Using whole genome sequencing a novel blaIMP gene, blaIMP-58 was discovered and characterized.
Insights
A multidrug-resistant Pseudomonas putida strain was found in a hospitalized patient. Whole genome sequencing identified a new blaIMP-58 gene, contributing to antibiotic resistance.
Area of Science:
- Microbiology
- Genomics
- Infectious Diseases
Background:
- Pseudomonas putida is an opportunistic pathogen.
- Multidrug resistance (MDR) in bacteria poses a significant global health threat.
- Urinary tract infections are common in hospitalized patients.
Observation:
- A 65-year-old female patient with severe clinical conditions presented with a urinary tract infection.
- The isolated Pseudomonas putida strain exhibited resistance to multiple antibiotics.
Findings:
- Whole genome sequencing was employed to analyze the MDR Pseudomonas putida isolate.
- A novel metallo-beta-lactamase gene, designated blaIMP-58, was discovered and characterized.
- The blaIMP-58 gene confers resistance to a broad spectrum of beta-lactam antibiotics.
Implications:
- The discovery of blaIMP-58 expands the known repertoire of antibiotic resistance genes.
- This finding highlights the importance of genomic surveillance for identifying emerging resistance mechanisms.
- Understanding novel resistance genes is crucial for developing effective therapeutic strategies against MDR infections.
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