Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Titrimetric Methods: Types and Commonly Used Strategies01:08

Titrimetric Methods: Types and Commonly Used Strategies

2.7K
In chemistry, titrimetric methods are broadly classified into three types: volumetric, gravimetric, and coulometric. Volumetric titrations involve measuring the volume of a titrant of known concentration that is required to react completely with an analyte. In gravimetric titrations, the standard solution reacts with the analyte to form an insoluble precipitate, which is filtered, dried, and weighed. In coulometric titrations, current is applied to an electrochemical reaction until the reaction...
2.7K
EDTA: Indirect and Alkalimetric Titration01:23

EDTA: Indirect and Alkalimetric Titration

2.0K
Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
2.0K
Potentiometric Titration: Overview01:31

Potentiometric Titration: Overview

5.1K
Potentiometric titration is a quantitative analytical technique that determines the concentration of an analyte by measuring the potential difference between the two electrodes in the solution. The endpoint of a potentiometric titration is the point at which there is a significant change in the potential difference. It occurs when the stoichiometric reaction between the analyte and the titrant is complete. The endpoint is usually determined graphically by plotting the measured potential...
5.1K
Classification of Titrimetric Analysis Based on Reaction Types01:01

Classification of Titrimetric Analysis Based on Reaction Types

1.9K
Titrimetric analysis in solution chemistry involves measuring the volume of solutions and is often called volumetric analysis. The standard solution of known concentration in the burette is called the titrant, whereas the solution of unknown concentration in the flask is called the analyte, or titrand. Titrimetric analyses can be classified into four types based on the reactions between the titrant and analyte.
Titrations between an acid and a base lead to neutralization reactions that form...
1.9K
Conductometric Titrations: Strong Acid-Base and Weak Acid-Base Titrations01:29

Conductometric Titrations: Strong Acid-Base and Weak Acid-Base Titrations

27
In acid-base titrations, conductance measurements are utilized to detect the endpoint. This method is grounded on the fact that electrical conductance relies on the number and mobility of ions. For instance, consider titrating strong acid HCl with a strong NaOH base. Initially, the HCl in the conductivity vessel conducts electricity due to the presence of hydrogen ions and chloride ions. As NaOH is gradually added from the burette, the fast-moving hydrogen ions are replaced by slower-moving...
27
Complexometric Titration: Overview00:39

Complexometric Titration: Overview

12.8K
Complexometric titration involves the formation of a complex by reacting a metal ion with one or more ligands. A visual indicator often detects the end point of a complexometric titration. It is added to the metal solution before the titration, forming a stable metal–indicator complex and imparting color to the solution. As the titration approaches the equivalence point, the excess of the added ligand displaces the indicator from the metal–indicator complex, releasing the free...
12.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrative multi-omics profiling identifies a lactylation-associated, metabolically active, and immunosuppressive subtype of colon adenocarcinoma.

Molecular & cellular oncology·2026
Same author

[Analysis of Abnormal Genotyping at <i>Amelogenin</i> Locus in Male Individuals].

Fa yi xue za zhi·2026
Same author

Enhanced stability of immobilized xylanase with nano‑zinc oxide/ethyl cellulose composite carrier for efficient xylan hydrolysis.

Food chemistry·2026
Same author

Citric Acid-Modified Sweet Potato Residue Insoluble Dietary Fiber as a Stabilizer for Pickering Emulsions.

Journal of food science·2026
Same author

Distinct hydrolyzed Al-Ti species govern the coagulation behavior and subsequent anaerobic fermentation of CEPS sludge.

Water research·2026
Same author

An in silico evaluation of tri-allelic SNPs from 1kGP Phase IV data for distant kinship identification and mixture analysis.

Forensic science international. Genetics·2026

Related Experiment Video

Updated: Mar 13, 2026

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
07:14

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

12.1K

Conductometric titration to determine total volatile basic nitrogen (TVB-N) for post-mortem interval (PMI).

Zhiyuan Xia1, Xiandun Zhai1, Beibei Liu1

  • 1Forensic Medicine School, Henan University of Science and Technology, No. 31 Anhui Road, Jianxi District, Luoyang, Henan 471003, China.

Journal of Forensic and Legal Medicine
|October 25, 2016
PubMed
Summary

This study introduces a new method using total volatile basic nitrogen (TVB-N) to measure cadaver decomposition rates for post-mortem interval (PMI) estimation. The developed technique is accurate and rapid, offering a practical approach for forensic science.

Keywords:
Conductometric titrationDecomposition rateForensic sciencePost-mortem interval estimationTotal volatile basic nitrogen

More Related Videos

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.7K
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.9K

Related Experiment Videos

Last Updated: Mar 13, 2026

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx
07:14

Automated, High-resolution Mobile Collection System for the Nitrogen Isotopic Analysis of NOx

Published on: December 20, 2016

12.1K
Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

6.7K
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
08:23

Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds

Published on: February 16, 2022

4.9K

Area of Science:

  • Forensic Science
  • Biochemistry
  • Analytical Chemistry

Background:

  • Accurate post-mortem interval (PMI) estimation relies on precise measurement of cadaver decomposition rates.
  • Existing methods for decomposition rate measurement are insufficient for reliable PMI estimation.
  • Total volatile basic nitrogen (TVB-N), a known indicator of meat freshness, has potential for assessing decomposition.

Purpose of the Study:

  • To establish a practical and accurate method for determining TVB-N in cadaver soft tissues.
  • To utilize TVB-N as an indicator for measuring decomposition rates and aiding PMI estimation.
  • To investigate the relationship between TVB-N levels and post-mortem interval (PMI).

Main Methods:

  • TVB-N determination in simulated and animal experiments using steam distillation and conductometric titration.
  • Kjeldahl distillation unit and conductivity meter were employed for precise measurements.
  • Validation involved ammonium standards and skeletal muscle tissue from rats over 312 hours at 24°C.

Main Results:

  • The TVB-N determination method demonstrated high precision with 99%-100% nitrogen recovery rates.
  • TVB-N levels in skeletal muscle showed significant changes with PMI, particularly after 24 hours.
  • A strong correlation was found between TVB-N and PMI (y = 3.35E-5x³ - 2.17E-2x² + 6.13x - 85.82) and initial electrical conductivity (y = 1.98x + 16.16).

Conclusions:

  • The developed TVB-N measurement method is accurate, rapid, and flexible for assessing cadaver decomposition.
  • This technique can serve as a foundational method for future PMI estimation research.
  • Further studies are recommended to validate these findings in diverse forensic contexts.