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Antibiotic sensitivity of enterobacteriaceae by disc diffusion and rapid fermentation sensitivity test methods
Indian Journal of Pathology & Microbiology
|July 1, 1989
Summary
This study compared conventional disc diffusion (DD) with rapid fermentation sensitivity testing (RFST) for antibiotic susceptibility in Gram-negative bacilli. RFST offers a faster, simpler alternative for immediate antibiotic sensitivity results, despite some limitations.
Area of Science:
- Clinical Microbiology
- Bacteriology
- Antimicrobial Susceptibility Testing
Background:
- Gram-negative bacilli are common causes of infections.
- Accurate and rapid antibiotic susceptibility testing is crucial for effective treatment.
- Conventional methods can be time-consuming.
Purpose of the Study:
- To compare the conventional disc diffusion (DD) method with the rapid fermentation sensitivity test (RFST) for determining antibiotic susceptibility.
- To evaluate the correlation and practicality of RFST for common Gram-negative bacilli.
Main Methods:
- 400 strains of Gram-negative bacilli were tested.
- Methods used: conventional disc diffusion (DD) and rapid fermentation sensitivity test (RFST).
- Antibiotics tested: streptomycin, chloramphenicol, kanamycin, gentamycin, and ampicillin.
Main Results:
- Correlation between DD and RFST ranged from 75% to 98%.
- RFST provided results within six hours.
- RFST is simple, inexpensive, rapid, and reliable, suitable for small laboratories.
Conclusions:
- RFST is a valuable tool for rapid antibiotic sensitivity testing, especially when immediate results are needed.
- Limitations include inability to test certain bacteria (e.g., Pseudomonas aeruginosa) or antibiotics (e.g., tetracycline) and inability to detect partial sensitivity.
- Despite limitations, RFST is a practical and helpful method for clinical laboratories.