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Identification of an immunodominant antigenically conserved 32-kilodalton protein from Cowdria ruminantium

F Jongejan1, M J Thielemans

  • 1Department of Tropical Veterinary Medicine and Protozoology, Institute of Infectious Diseases and Immunology, Utrecht, The Netherlands.

Infection and Immunity
|October 1, 1989
PubMed

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Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.