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Updated: Mar 13, 2026

Micro-dissection of Enamel Organ from Mandibular Incisor of Rats Exposed to Environmental Toxicants
Published on: March 29, 2018
A comparative study of the susceptibility of cut and uncut enamel to erosive demineralization
Wa Than Lin1, Yuichi Kitasako, Syozi Nakashima
1Cariology and Operative Dentistry, Department of Restorative Sciences, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University.
Abstract:
This study aimed to evaluate the susceptibility of cut and uncut enamel surfaces to an erosive challenge and to examine the resultant characteristics/morphological changes. Ten extracted human incisors were used for preparation of enamel samples, and samples were immersed in citric acid. After 3 (total 3 min) and 6 cycles (total 6 min) of erosive challenges, surface loss (SL) and morphological changes were measured using scanning microscopy and FIB-TEM. Ca release (CA) and surface hardness (SH) were measured using a calcium-sensitive electrode and hardness tester respectively. Mean values of all measurements were statistically analyzed by using a t-test. Uncut enamel samples had significantly lower SL and greater SH than cut enamel (p<0.01). Cut enamel samples after 3 cycles showed higher CA compared with those from uncut enamel samples (p<0.05). Cut enamel was shown to be more susceptible to acidic dissolution and deeper acid penetration than uncut enamel after erosive demineralization.

